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Friday, 25 August

23:51

Food Forests: The Perfect Match for Reluctant Gardeners Natural Blaze

Dont have a green thumb? Try out this low-maintenance and high-yield technique.

The number of Americans producing their own fruits and vegetables has increased more than 25 percent since 2008, growing to 44 million of our friends and neighbors. Yet, many reject growing their own food, worried that home gardening is too large a time, labor, and monetary commitment. Hopefully, the concept of a self-sustaining food forest will inspire people to reconsider their perceptions and investigate how they could make growing food a part of their lives.

So, what is a food forest? A food forest is an agricultural concept that takes root in permaculture, a practice that promotes self-sustaining, food-producing ecosystems by emphasizing perennial, low-maintenance indigenous crops that can survive off of natural nutrient inputs, drainage patterns, and climate.

Food forests require little maintenance, but are very fruitful; a perfect combination for the reluctant home gardener.

...

22:30

Co-author of now-retracted paper on shooter video games may have PhD revoked Retraction Watch

A researcher who co-authored a paper about video games that was retracted earlier this year may lose her PhD from The Ohio State University. As The Columbus Dispatch reported yesterday, the universitys board of trustees is set to vote today on whether to revoke Jodi Whitakers degree. While a graduate student at Ohio State, Whitaker []

The post Co-author of now-retracted paper on shooter video games may have PhD revoked appeared first on Retraction Watch.

22:30

Is the use of Open-Label Placebo Ethical in the Treatment of Children? Science-Based Medicine

Is the use of "open-label" placebo ethical in pediatric medical care, or any care for that matter? A recent article in Pediatrics discussing this issue comes to a flawed conclusion based on a misunderstanding of placebo and of the literature on placebo without deception.

20:33

A Mad World: Capitalism and the Rise of Mental Illness Mad In America

From Red Pepper: Capitalism produces much of the mental distress that is categorized as mental illness by turning human creativity and connectivity into social isolation, alienation, and objectification.

Many people believe, and are encouraged to believe, that these problems and disorders psychosis, schizophrenia, anxiety, depression, self-harm these symptoms of a sick world (to use James Hillmans terrific description) are theirs, rather than the worlds. But what if your emotional problems werent merely your own?, asks Tom Syverson. What if they were our problems? What if the real problem is that were living in wrong society? Perhaps Adorno was correct when he said, wrong life cannot be lived rightly.

The root of this living wrongly seems to be because we live in a social and economic system at odds with both our psychology and our neurology, with who we are as social beings. As I suggest in my book, we need to realise that our inner and outer worlds constantly and profoundly interact and shape each other, and that therefore rather than separating our understanding of economic and social practices from our understanding of psychology and human development, we need to bring them together, to align them. And for this to happen, we need a new dialogue between the political and personal worlds, a new integrated model for mental health, and a new politics.

Article

20:32

Tripping for Knowledge: The Psychedelic Epistemologist Mad In America

In this interview for 3:AM Magazine, Chris Letheby argues that even though psychedelics may induce delusions or imaginary phenomena, their use can also lead to accurate knowledge and insights into ones own mind and consciousness. He also discusses some of the key differences between antidepressant use, which can be dehumanizing, and psychedelic use, which is often transformative.

I just think that our intuitive conception of how drugs like SSRIs work fits the description of what some thinkers regard as a dehumanising treatment modality. Whats the description? Basically, they re-wire you from the bottom up, and you, as a person, dont really have anything to do with it. The change in personality or mood is alienits inserted from the outside of ones biography, by the drug, rather than being a meaningful and comprehensible change that one goes through and can incorporate into ones autobiography. This contrasts starkly with prototypical psychotherapies, be it cognitive-behavioural therapy, Freudian analysis, exposure therapy, or whatever, where there is a sense that the transformation results from learning and insight, and the subject, post-transformation, can tell a plausible story about how and why that particular experience or insight transformed them in the way that it did.

Well, a glance at the literature on psychedelic therapy, and in particular the reports of psychedelic subjects, gives you a picture much more like the second than the first. People are talking about being transformed by meaningful learning experiences, and typically they can tell a story about why that particular experience transformed them in that particular way. Of course, they might be confabulating. But I think its plausible that psychedelic therapy does in fact involve something more like the intuitive picture of psychotherapy than like the intuitive picture of pharmacotherapyexplicable transformation by a meaningful conscious experience. A philosophical slogan would be that psychedelic therapy is a personal-level process.

20:31

Indescribable You Mad In America

In this piece for Aeon, Carlin Flora critiques sociologists and psychologists attempts to categorize and classify peoples personalities into distinct groups and profiles, advocating that we instead recognize the fluidity and complexity of our personalities.

were constantly describing ourselves and others. Sometimes, the stakes are low, as with idle chat, and sometimes they are higher, such as when were summing up a potential job candidate or a possible love match for a friend. Writers search for emotional granularity, consequential details and apt metaphors, while sociologists and personality psychologists have come up with sorting tools such as the Big Five personality traits extraversion, neuroticism, agreeableness, openness to experience, and conscientiousness. But if I tell you someone is a nice-looking middle-aged Asian extravert who is fairly agreeable, neurotic and open-minded, and from the Midwest you have no idea what shes really like. You barely have a starting point.

People have habits of speech, mannerisms, a temperament that is at least in part inborn, and even behavioural signatures and routines. But across time and contexts, any of these characteristics can change. We can act against our own proclivities until they arent proclivities anymore. Being enclosed in solid, distinct bodies fuels the belief that our personalities must be fully formed and consistent as well. But they arent. Everyone from pop-psych authors to business-school professors to astrologers has come up with her own system for sizing up people. If it were possible, wouldnt one method have prevailed by now? In fact, two recent paradigm-breaking studies suggest that personality traits can shift slowly yet drastically over time, and quite quickly after therapeutic interventions.

20:31

More Denial: Some Autism Parents Are Just ASDholes AGE OF AUTISM

Below is a horrible story of a mother charged with murdering her teen daughter with "high functioning autism."The girl's name was Savannah Leckie. According to the article, it sounds like the girl was more than a handful - and don't...

17:50

Bursts of Endorphins Are Released With This One Type of Exercise PreventDisease.com

Researchers in Finland have found that when youre working out, the level of endoprhins released in the brain directly correlates to the intensity of exercise youre doing. Thats a good thing for people doing high intensity interval training, or HIIT, because HIIT can be miserable.

13:15

There is a large body of preclinical evidence in support of cannabinoids as potential analgesic agents. GreenMedInfo

PMID:  Adv Pharmacol. 2017 ;80:437-475. Epub 2017 Jun 20. PMID: 28826543 Abstract Title:  Cannabinoids and Pain: Sites and Mechanisms of Action. Abstract:  The endocannabinoid system, consisting of the cannabinoid1 receptor (CB1R) and cannabinoid2 receptor (CB2R), endogenous cannabinoid ligands (endocannabinoids), and metabolizing enzymes, is present throughout the pain pathways. Endocannabinoids, phytocannabinoids, and synthetic cannabinoid receptor agonists have antinociceptive effects in animal models of acute, inflammatory, and neuropathic pain. CB1R and CB2R located at peripheral, spinal, or supraspinal sites are important targets mediating these antinociceptive effects. The mechanisms underlying the analgesic effects of cannabinoids likely include inhibition of presynaptic neurotransmitter and neuropeptide release, modulation of postsynaptic neuronal excitability, activation of the descending inhibitory pain pathway, and reductions in neuroinflammatory signaling. Strategies to dissociate the psychoactive effects of cannabinoids from their analgesic effects have focused on peripherally restricted CB1R agonists, CB2R agonists, inhibitors of endocannabinoid catabolism or uptake, and modulation of other non-CB1R/non-CB2R targets of cannabinoids including TRPV1, GPR55, and PPARs. The large body of preclinical evidence in support of cannabinoids as potential analgesic agents is supported by clinical studies demonstrating their efficacy across a variety of pain disorders.

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12:21

Are you promoting Dr. Sebi? Audio 14 Advocates for Dr. Sebi

Have you heard about the guy who was curing all sorts of diseases with natural herbs? The same guy who won the case in the supreme court of New York proving the he cures the incurables! Why wasnt he as popular as he should have been? Long story short, it Continue reading

12:18

These results suggest that lower doses of curcumin can influence several steps involved in tumorigenesis. GreenMedInfo

PMID:  Phytother Res. 2017 Aug 7. Epub 2017 Aug 7. PMID: 28782139 Abstract Title:  Low Doses of Curcuma longa Modulates Cell Migration and Cell-Cell Adhesion. Abstract:  Cell invasion and metastasis are involved in clinical failures in cancer treatment, and both events require the acquisition of a migratory behavior by tumor cells. Curcumin is a promising natural product with anti-proliferative activity, but its effects on cell migration are still unclear. We evaluated the effects of curcumin on the proliferation, apoptosis, migration, and cell-cell adhesion of keratinocyte, oral squamous cell carcinoma (OSCC), and fibroblast cell lines, as well as in a xenograft model of OSCC. Curcumin (2 M) decreased cell proliferation in cell lines with mesenchymal characteristics, while cell death was detected only at 50 M. We observed that highly migratory cells showed a decrease on migration speed and directionality when treated with 2 or 5 M of curcumin (50% and 40%, respectively, p 

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11:34

Wogonin induces apoptosis of human osteosarcoma cancer stem cells. GreenMedInfo

PMID:  BMC Complement Altern Med. 2017 Jun 12 ;17(1):304. Epub 2017 Jun 12. PMID: 28606135 Abstract Title:  Wogonin suppresses stem cell-like traits of CD133 positive osteosarcoma cell via inhibiting matrix metallopeptidase-9 expression. Abstract:  BACKGROUND: Several efforts have been deployed to cure osteosarcoma, a high-grade malignant bone tumour in children and adolescents. However, some challenges such as drug resistance, relapse, and tumour metastasis remain owing to the existence of cancer stem cells (CSC). There is an urgent need to develop cost-effective and safe therapies.METHODS: Wogonin, an extract from the root of Scutellaria baicalensis, has long been considered as a promising natural and safe compound for anti-tumourigenesis, particularly to inhibit tumour invasion and metastasis. Hoechst 33,342 staining, wound healing assay, sphere formation assay, western blotting, and gelatin zymography assays were performed in CD133 positive osteosarcoma cell.RESULTS: In this study, we examined the effect of Wogonin on the mobility of human osteosarcoma CSC. Wogonin induces apoptosis of human osteosarcoma CSC, inhibits its mobility in vitro via downregulation of MMP-9 expression, and represses its renewal ability.CONCLUSIONS: We demonstrated that Wogonin decreases the renewal capacity of CSC. By inhibiting the formation of and reducing the size of spheres, Wogonin at a concentration of 40-80 M effectively minimizes potential risk from CSC. Taken together, we have demonstrated a new approach for developing a potential therapy for osteosarcoma.

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11:22

Wogonin could be an ideal candidate for increasing sorafenibs activity in hepatocellular carcinoma therapy. GreenMedInfo

PMID:  Oncol Lett. 2017 Jun ;13(6):5028-5034. Epub 2017 Apr 20. PMID: 28599504 Abstract Title:  Combination of wogonin and sorafenib effectively kills human hepatocellular carcinoma cells through apoptosis potentiation and autophagy inhibition. Abstract:  The small molecule multi-kinase inhibitor sorafenib has become the standard systemic treatment for patients with advanced hepatocellular carcinoma (HCC) and renal cell carcinoma. Similar to other kinase inhibitors, drug resistance hinders its clinical use; thus, combination therapy to improve sorafenib sensitivity is a promising approach. The present study shows for the first time that the combination of sorafenib and wogonin exerts a significant potentiation of cytotoxicity in a number of human HCC cell lines in a dose-dependent manner. Enhanced cell death was due to potentiation of apoptosis, which was demonstrated by increased apoptotic cell populations, caspase activation and suppression of cell death by the pan-caspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl. Sorafenib induced autophagy activation, which was shown by autophagic flux. Suppression of autophagy with the autophagy inhibitors chloroquine or 3-methyladenine significantly enhanced cytotoxicity, suggesting that sorafenib-induced autophagy is cytoprotective. Notably, wogonin effectively inhibited sorafenib-induced autophagy. Altogether, our results indicate that the combination of wogonin and sorafenib effectively kills human HCC cells. This occurs, at least in part, through autophagy inhibition, which potentiates apoptosis. Thus, wogonin could be an ideal candidate for increasing sorafenibs activity in HCC therapy, which warrants further investigation in vivo.

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11:08

Wogonin attenuates inflammation by activating PPAR- in alcoholic liver disease. GreenMedInfo

PMID:  Int Immunopharmacol. 2017 Sep ;50:95-106. Epub 2017 Jun 21. PMID: 28646664 Abstract Title:  Wogonin attenuates inflammation by activating PPAR- in alcoholic liver disease. Abstract:  Alcoholic liver disease (ALD) is one of the predominant causes of liver-related morbidity and mortality worldwide. However, effective therapy for ALD is still lacking. Wogonin, a major flavonoid compound, is found in Scutellaria baicalensis Georgi. Accumulating studies have revealed that wogonin possesses anti-inflammatory and anti-tumour activities in various models. However, the hepatoprotective activity of wogonin in ALD is still obscure. In this study, we found that wogonin significantly attenuated inflammatory response in EtOH-fed mice, and reduced the expression of inflammatory cytokines such as TNF- and IL-6 in EtOH-induced RAW264.7 cells. Furthermore, our findings showed that wogonin remarkably induced the expression of PPAR- in vivo and in vitro. Compared with the wogonin-treated group, blockade of PPAR- with inhibitor (T0070907) or PPAR- small interfering (si)-RNA were applied in RAW264.7 cells to evaluate the involvement of wogonin in alleviating EtOH-induced inflammation. Moreover, forced expression of PPAR- further suppressed the expression of TNF- and IL-6 when treated with wogonin on EtOH-induced RAW264.7 cells. In addition, it was demonstrated that wogonin remarkablysuppressed PPAR--meditated phosphorylation and activation of NF-B-P65. In conclusion, our results indicated that wogonin may serve as an effective modulator of PPAR- by down-regulating NF-B pathway, thereby attenuated inflammatory response in ALD.

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10:41

Wogonin intercalates with genomic DNA and exhibits protective effects in IL-1 stimulated osteoarthritis chondrocytes. GreenMedInfo

PMID:  Chem Biol Interact. 2017 Aug 25 ;274:13-23. Epub 2017 Jul 5. PMID: 28688942 Abstract Title:  Wogonin, a natural flavonoid, intercalates with genomic DNA and exhibits protective effects in IL-1 stimulated osteoarthritis chondrocytes. Abstract:  Wogonin has recently been shown to possess anti-inflammatory and chondroprotective properties and is of considerable interest due to its broad pharmacological activities. The present study highlights that Wogonin binds DNA and exerts chondroprotective effects in vitro. Wogonin showed strong binding with chondrocytes genomic DNA in vitro. The mode of binding of Wogonin to genomic-DNA was assessed by competing Wogonin with EtBr or DAPI, known DNA intercalator and a minor groove binder, respectively. EtBr fluorescence reduced significantly with increase inWogonin concentration suggesting possible DNA intercalation of Wogonin. Further, in silico molecular docking of Wogonin on mammalian DNA also indicated possible intercalation of Wogonin with DNA. The denaturation and FRET studies revealed that Wogonin prevents denaturation of DNA strands and providestability to genomic DNA against a variety of chemical denaturants. The cellular uptake study showed that Wogonin enters osteoarthritis chondrocytes and was mainly localized in the nucleus. Wogonin treatment to OA chondrocytes protects the fragmentation of genomic DNA in response to IL-1 as evaluated by DNA ladder and TUNEL assay. Treatment of chondrocytes with Wogonin resulted in significant suppression of IL-1-mediated induction of ROS. Further, Wogonin exhibited protective potential through potent suppression of extrinsic and intrinsic apoptotic pathways and induction of anti-apoptoticproteins in IL-1-stimulated osteoarthritis chondrocytes. Our data thus suggest that DNA intercalation by Wogonin may result in the stabilization of genomic DNA leading to protective activity.

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10:27

wogonin may be a promising multifunctional drug candidate for Alzheimer's disease. GreenMedInfo

PMID:  Evid Based Complement Alternat Med. 2017 ;2017:3545169. Epub 2017 Jun 7. PMID: 28680449 Abstract Title:  Protective Effects of Wogonin against Alzheimer's Disease by Inhibition of Amyloidogenic Pathway. Abstract:  One of the pathogenic systems of Alzheimer's disease (AD) is the formation of-amyloid plaques in the brains of patients, and amyloidogenic activity becomes one of the therapeutic targets. Here, we report wogonin, one of the major active constituting components in Scutellaria baicalensis, which has the neuroprotective effects on amyloid- peptides- (A-) induced toxicity.Oral wogonin treatment improved the performance of triple transgenic AD mice (h-APPswe, h-Tau P301L, and h-PS1 M146V) on the Morris water maze, Y-maze, and novel object recognition. Furthermore, wogonin activated the neurite outgrowth of AD cells by increasing neurite length and complexity of Tet-On A42-GFP SH-SY5Y neuroblastoma cells (AD cells) and attenuated amyloidogenic pathway by decreasing the levels of -secretase, APP -C-terminal fragment, A-aggregation, and phosphorylated Tau. Wogonin also increased mitochondrial membrane potential (m) and protected against apoptosis by reducing the expression of Bax and cleaved PARP. Collectively, these results conclude that wogonin may be a promising multifunctional drug candidate for AD.

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10:11

Phytochemical modulation of apoptosis and autophagy could be used to overcome chemoresistance in leukemic stem cells. GreenMedInfo

PMID:  Int Rev Neurobiol. 2017 ;135:249-278. Epub 2017 Apr 12. PMID: 28807161 Abstract Title:  Phytochemical Modulation of Apoptosis and Autophagy: Strategies to Overcome Chemoresistance in Leukemic Stem Cells in the Bone Marrow Microenvironment. Abstract:  Advances in scientific research and targeted treatment regimes have improved survival rates for many cancers over the past few decades. However, for some types of leukemia, including acute lymphoblastic and acute myeloid leukemia, mortality rates have continued to rise, with chemoresistance in leukemic stem cells (LSCs) being a major contributing factor. Most cancer drug therapies act by inducing apoptosis in dividing cells but are ineffective in targeting quiescent LSCs. Niches in the bone marrow, known as leukemic niches, behave as"sanctuaries"where LSCs acquire drug resistance. This review explores the role of the bone marrow environment in the maintenance of LSCs and its contribution to chemoresistance and considers current research on the potential use of phytochemicals to overcome chemoresistance through the modulation of signaling pathways involved in the survival and death of leukemic clonal cells and/or leukemic stem cells. Phytochemicals from traditional Chinese medicine, namely baicalein, chrysin, wogonin (constituents of Scutellaria baicalensis; hung qn; ), curcumin (a constituent of Curcuma longa, jing hung, ), and resveratrol (a constituent of Polygonum cuspidatum; h zhng, ) have been shown to induce apoptosis in leukemic cell lines, with curcumin and resveratrol also causing cell death via the induction of autophagy (a nonapoptotic pathway). In order to be effective in eliminating LSCs, it is important to target signaling pathways (such as Wnt/-catenin, Notch, and Hedgehog). Resveratrol has been reported to induce apoptosis in leukemic cells through the inhibition of the Notch and Sonic hedgehog signaling pathways, therefore showing potential to affect LSCs. While these findings are of interest, there is a lack of reported research on the modulatory effect of phytochemicals on the autophagic cell death pathway in leukemia, and on the signaling pathways involved in the maintenance of LSCs, highlighting the need forfurther work in these areas.

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09:52

Call to Stop Using Africans As GMO Guinea Pigs Natural Blaze

By Heather CallaghanEditor

The Alliance for Food Sovereignty in Africa is calling for an immediate halt on imports to South Africa of Monsantos high-risk second-generation gene-silencing GM maize meant for human consumers.

African Center for Biodiversity reports:

In an open letter to African biosafety regulators, AFSA rejects and condemns US corporation Monsantos plan to exploit millions of Africans as unwitting human guinea pigs for their latest genetic engineering experiment. AFSA also condemns the IITA (International Institute of Tropical Agriculture) field trial application in Nigeria using this same risky technology to produce GM cassava for the agro-fuels industry.

These GM applications target staple foods of maize and cassava, eaten by many millions of Africans every day. AFSAs open letter states, Scientists have reported that the untested gene-silencing effect is able to cross over into mammals and humans, and affect their genetic makeup with unknown potential negative consequences, and have called for long-term animal testing and stronger regulation before this goes ahead.

The technology that the AFSA is referring to is the controversial RNAi. They are demanding the use of RNAi tech to be adamantly banned while the risks remain and for regulators in South Africa and Nigeria to reject the imp...

09:45

This study demonstrated the regulative effect of curcumin administration on gut microbiota in mice. GreenMedInfo

PMID:  Food Nutr Res. 2017 ;61(1):1361780. Epub 2017 Aug 9. PMID: 28814952 Abstract Title:  Regulative effects of curcumin spice administration on gut microbiota and its pharmacological implications. Abstract:  Curcumin, the major active component of turmeric (Curcuma longa), is widely used as a spice and food-coloring agent, and also exhibits multiple biological activities. However, as curcumin has poor systemic bioavailability its pharmacology remains to be elucidated. Owing to the high concentration of curcumin in the gastrointestinal tract after oral administration, we hypothesize that it may exert regulative effects on the gut microbiota. We investigated the regulative effects of oral curcumin administration on the gut microbiota of C57BL/6 mice and found that curcumin significantly affected the abundance of several representative families in gut microbial communities, including Prevotellaceae, Bacteroidaceae, and Rikenellaceae. Considering the pathogenic associations between gut microbiota and many diseases, the present findings may help us to interpret the therapeutic benefits of curcumin.

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09:44

Share this Link, Save the Future of 2025! BolenReport

By Kent Heckenlively, JD Senior MIT Research Scientist, Dr. Stephanie Seneff has estimated that if current trends continue, half of all children born in 2025 and afterwards will develop autism. I cannot imagine any scenario in which this does not bring about the eventual collapse of the human species.  My FIVE YEAR CHILDHOOD VACCINE MORATORIUM Continue reading Share this Link, Save the Future of 2025!

The post Share this Link, Save the Future of 2025! appeared first on BolenReport.

09:33

Simplest Envelope Budget Method Youve Never Seen Before Natural Blaze

By Natural Blaze via FunCheapOrFree

Youve heard of using envelopes for budgeting, right?

Its a basic tip many financial gurus insist their readers do. It means no using credit cards, only allotted amounts of cash that you pull from labeled envelopes. If you go to the grocery store, you can only use the money thats meant for the grocery store and when its gone, tough luck!

Its okay in theory, but I think we all know what happens. We rob Peter to pay Paul we take from the other envelopes and end up with multiple deficits. We feel deprived and abandon the budget.

Jordan Page from Fun Cheap or Free came up with a nifty method using an old envelope that isnt quite what you think.

Watch and tell us what you think:

 

YouTube: https://www.youtube.com/watch?v=VCr-54OH7IY

Description:

Simplest budgeting youll ever do...

09:30

The results demonstrated a marked efficacy of curcumin in experimental model of Parkinson's disease. GreenMedInfo

PMID:  BMC Complement Altern Med. 2017 Aug 17 ;17(1):412. Epub 2017 Aug 17. PMID: 28818104 Abstract Title:  Neuroprotective properties of curcumin in toxin-base animal models of Parkinson's disease: a systematic experiment literatures review. Abstract:  BACKGROUND: Curcumin (diferuloylmethane), a polyphenol extracted from the plant Curcuma longa, is widely used in Southeast Asia, China and India in food preparation and for medicinal purposes. Meanwhile, the neuroprotective actions of curcumin have been documented for experimental therapy in Parkinson's disease (PD).METHODS: In this study, we used a systematic review to comprehensively assess the efficacy of curcumin in experimental PD. Using electronic and manual search for the literatures, we identified studies describing the efficacy of curcumin in animal models of PD.RESULTS: We identified 13 studies with a total of 298 animals describing the efficacy of curcumin in animal models of PD. The methodological quality of all preclinical trials is ranged from 2 to 5. The majority of the experiment studies demonstrated that curcumin was more significantly neuroprotection effective than control groups for treating PD. Among them, five studies indicated that curcumin had an anti-inflammatory effect in the PD animal models (p 

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09:19

Metformin in combination with curcumin inhibits the growth, metastasis and angiogenesis of hepatocellular carcinoma. GreenMedInfo

PMID:  Mol Carcinog. 2017 Aug 19. Epub 2017 Aug 19. PMID: 28833603 Abstract Title:  Metformin incombination with curcumin inhibits the growth?metastasis and angiogenesis of hepatocellular carcinoma in vitro and in vivo. Abstract:  Hepatocellular carcinoma (HCC) has poor prognosis due to the advanced disease stages by the time it is diagnosed, high recurrence rates and metastasis. In the present study, we investigated the effects of metformin (a safe anti-diabetic drug) and curcumin (a turmeric polyphenol extracted from rhizome of Curcuma longa Linn.) on proliferation, apoptosis, invasion, metastasis and angiogenesis of HCC in vitro and in vivo. It was found that co-treatment of metformin and curcumin could not only induce tumor cells into apoptosis through activating the mitochondria pathways, but also suppress the invasion, metastasis of HCC cells and angiogenesis of HUVECs. These effects were associated with downregulation of the expression of MMP2/9, VEGF and VEGFR-2, up-regulation of PTEN, P53 and suppression of PI3K/Akt/mTOR/NF-B and EGFR/STAT3 signaling. Co-administration of metformin and curcumin significantly inhibited HCC tumor growth than administration with metformin or curcumin alone in a xenograft mouse model. Thus, metformin and curcumin in combination showed a better anti-tumor effects in hepatoma cells than either metformin or curcumin presence alone and might represent an effective therapeutic strategy for HCC treatment. This article is protected by copyright. All rights reserved.

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09:17

Flu Shots new research findings two

  • The Disgusting Stuff in Flu Shots

POSTED BY: SHERRI TENPENNY, DO 08/23/2017
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IMPORTANT NOTE: Dr. Tenpenny has JUST released her newest eBook. CHALLENGING THE VACCINE DOGMA. I encourage everyone to join the Vaxxter newsletter and get your FREE copy before it is gone. Begin MailChimp Signup Form
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By Dr. Sherri Tenpenny, DO, AOBNMM, ABIHM
Its that time again: the hype has begun to push flu shots into the arms of everyone, from six-month-old infants to 96-year-old grandparents. Flu vaccines have been around since the 1930s when it was discovered that the influenza viruses grew well in eggs and could be harvested for a vaccine. Flu shots contain stray, potentially cancer-causing stray virusesand the multi-dose vials remain loaded with mercury. For nearly 90 years, this disgusting slurry has annually been injected into humans. Truth is, the long-term consequences of this action are completely unknown.
For the 2017-18 flu season, there will be a variety of 10 different flu shots made by five different manufacturers:
  • Seqirus: Afluria, Fluad, FluVirin, FlucelVax
  • Protein Sciences: FluBlok
  • MedImmune: FluMist, (a...

09:01

Fermented C. longa might be a useful material for preventing impairment of learning and memory. GreenMedInfo

PMID:  BMC Complement Altern Med. 2017 Jul 17 ;17(1):367. Epub 2017 Jul 17. PMID: 28716085 Abstract Title:  The protective effect of fermented Curcuma longa L. on memory dysfunction in oxidative stress-induced C6 gliomal cells, proinflammatory-activated BV2 microglial cells, and scopolamine-induced amnesia model in mice. Abstract:  BACKGROUND: Curcuma longa L. is a well-known medicinal plant that has been used for its anti-cancer, neuroprotective, and hepatoprotective effects. However, the neuroprotective effect of fermented C. longa (FCL) has not been reported. Therefore, in this study, the effectiveness of FCL for the regulation of memory dysfunction was investigated in two brain cell lines (rat glioma C6 and murine microglia BV2) and scopolamine-treated mice.METHODS: C. longa powder was fermented by 5% Lactobacillus plantarum K154 containing 2% (w/v) yeast extract at 30 C for 72 h followed by sterilization at 121 C for 15 min. The protective effects of fermented C. longa (FCL) on oxidative stress induced cell death were analyzed by MTT assay in C6 cells. The anti-inflammatory effects of FCL were investigated by measuring the production of nitric oxide (NO)and prostaglandin E2 (PGE2) as well as the expression levels of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated BV2 cells. The step-through passive avoidance test, Morris water maze test, acetylcholinesterase (AChE) activity, and expression of cAMP response element-binding protein (CREB) and brain-derived neurotropic factor (BDNF) were employed to determine the effects of FCL on scopolamine-induced memory deficit in mice. The contents of curcuminoids were analyzed through LC/MS.RESULTS: Pretreatment with FCL effectively prevented the cell death induced by oxidative stress in C6 cells. Moreover, FCL inhibited the production NO and PGE2 via the inhibition of iNOS and COX-2 expression in BV2 cells. FCL significantly attenuated scopolamine-induced memory impairment in mice and prevented scopolamine-induced AChE activity in the hippocampus. Additionally, FCL reversed the reduction of CREB and BDNF expression. The curcuminoids content in FCL was 1.44%.CONCLUSION: FCL pretreatment could alleviate scopolamine-induced memory impairment in mice, as well as oxidative stress and inflammation in C6 and BV2 cells, respectively. Thus, FCL might be a useful material for preventing impairment of learning and memory.

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09:00

Who Will Pay The Ransom When Smart Meters Are Hacked Into? Natural Blaze

By Catherine J. Frompovich

Do you recall that a hospital actually paid hackers $17,000 ransom for the release of the hospitals data? That was the Hollywood Presbyterian Medical Center, which paid using Bitcoin in February 2016. Another hacked company didnt get off so cheaply! The South Korean web hosting company Nayana paid 397.6 Bitcoin, which was equivalent to almost a million dollars! Thats not all.

HealthcareITNews reported under a Privacy & Security heading, Ransomware: See the 14 hospitals attacked so far in 2016 [to October]. [1]

Renault factories in France and Slovenia were temporarily shut down in May 2017 due to a ransomware attack! Hackers are real, while hacking is big time stuff even for teens who want to exercise their cyberspace prowess or power-hungry antics.

Pharmaceutical giant, Merck & Company, was rocked by ransomware at its New Jersey headquarters in June 2017 by the WannaCry virus! Employees were told to close their computers and go home! [2]

In 2016, Forbes published that

...

08:47

Curcuminoids supplementation could contribute to a reduced risk of cardiovascular events in dyslipidemic patients with T2D. GreenMedInfo

PMID:  Complement Ther Med. 2017 Aug ;33:1-5. Epub 2017 May 29. PMID: 28735818 Abstract Title:  Curcuminoids modify lipid profile in type 2 diabetes mellitus: A randomized controlled trial. Abstract:  BACKGROUND: Type 2 diabetes (T2D) is an established risk factor for cardiovascular disease (CVD) and is associated with disturbed metabolism of lipids and lipoproteins. Curcuminoids are natural products with anti-diabetic and lipid-modifying actions but their efficacy in improving dyslipidemia in diabetic individuals has not been sufficiently studied.OBJECTIVE: To investigate the efficacy of supplementation with curcuminoids, plus piperine as an absorption enhancer, in improving serum lipids in patients with T2D.METHODS: In this 12-week randomized double-blind placebo-controlled trial, subjects with T2D (n=118) were assigned to curcuminoids (1000mg/day plus piperine 10mg/day) or placebo plus standard of care for T2D. Serum concentrations of lipids including total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), triglycerides (TG), lipoprotein(a) [Lp(a)], and non-HDL-C were determined at baseline and at the end of trial.RESULTS: Between-group comparison of change in the study parameters revealed significant reductions in serum levels of TC (-21.8625.78 versus -17.0641.51, respectively; p=0.023), non-HDL-C (-23.4225.13 versus -16.8441.42, respectively; p=0.014) and Lp(a) (-1.501.61 versus -0.341.73, respectively; p=0.001) and elevations in serum HDL-C levels (1.564.25 versus -0.224.62, respectively; p=0.048) in the curcuminoids group as compared with the placebo group (p0.05).CONCLUSION: Curcuminoids supplementation can reduce serum levels of atherogenic lipid indices including non-HDL-C and Lp(a). Therefore, curcuminoids supplementation could contribute to a reduced risk of cardiovascular events in dyslipidemic patients with T2D.

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08:37

Piperine inhibits Aflatoxin B1 production in Aspergillus flavus by modulating fungal oxidative stress response. GreenMedInfo

PMID:  Fungal Genet Biol. 2017 Aug 19. Epub 2017 Aug 19. PMID: 28830793 Abstract Title:  Piperine inhibits Aflatoxin B1 production in Aspergillus flavus by modulating fungal oxidative stress response. Abstract:  Aspergillus flavus, a soil-borne pathogen, represents a danger for humans and animals since it produces the carcinogenic mycotoxin Aflatoxin B1 (AFB1). Approaches aiming the reduction of this fungal contaminant mainly involve chemicals that may also be toxic. Therefore, identification and characterization of natural anti-aflatoxigenic products represents a sustainable alternative strategy. Piperine, a major component of black and long peppers, has been previously demonstrated as an AFB1-inhibitor; nevertheless its mechanism of action was yet to be elucidated. The aim of the present study was to evaluate piperine's molecular mechanism of action in A. flavus with a special focus on oxidative stress response. For that, the entire AFB1 gene cluster as well as a targeted gene-network coding for fungal stress response factors and cellular receptors were analyzed. In addition to this, fungal enzymatic activities were also characterized. We demonstrated that piperine inhibits aflatoxin production and fungal growth in a dose-dependent manner. Analysis of the gene cluster demonstrated that almost all genes participating in aflatoxin's biosynthetic pathway were down regulated. Exposure to piperine also resulted in decreased transcript levels of the global regulator veA together with an over-expression of genes coding for several basic leucine zipper (bZIP) transcription factors such as atfA, atfB and ap-1 and genes belonging to superoxide dismutase and catalase's families. Furthermore, this gene response was accompanied by a significant enhancement of catalase enzymatic activity. In conclusion, these data demonstrated that piperine inhibits AFB1 production while positively modulating fungal antioxidant status in A. flavus.

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08:30

Theres A Definite Discrimination Aspect To Pennsylvanias Smart Meter Law Natural Blaze

By Catherine J. Frompovich

Whats discrimination?

But in the context of civil rights law, unlawful discrimination refers to unfair or unequal treatment of an individual (or group) based on certain characteristics, including: [1] [CJF emphasis]

[U]nequal treatment of persons, for a reason which has nothing to do with legal rights or ability. Federal and state laws prohibit discrimination in employment, availability of housing, rates of pay, right to promotion, educational opportunity, civil rights, and use of facilities based on race, nationality, creed, color, age, sex or sexual orientation. The rights to protest discrimination or enforce ones rights to equal treatment are provided in various federal and state laws, which allow for private lawsuits with the right to damages. There are also federal and state commissions to investigate and enforce equal rights. [2] [CJF emphasis]

What are some synonyms for discrimination?

synonyms:

prejudice, bias, bigotry, intolerance, narrow-mindedness, unfairness, inequity, favoritism, one-sidedness, partisanship; [3]

How many types of discrimination are there?

08:13

Pedophile Farm Alleged in Lawsuit Against Washington Foster Care Home for Boys Health Impact News

Centralias Kiwanis Vocational Home News Stories

Chronicle headlines in the 1980s and 1990s alluded to trouble at the Kiwanis Vocational Home, but nothing as explosive as claims included in a number of lawsuits filed in relation to alleged abuse, negligence and fraud at the facility, which closed in 1994. Image Source.

By Natalie Johnson
The Chronicle

Excerpts:

Centralias Kiwanis Vocational Home, open from 1979 to 1994, was intended to be a safe place for wayward boys, a state-licensed foster home where 11- to 17-year-olds could get an education and job skills in a family atmosphere, according to a 1986 Chronicle article.

However, four lawsuits from former residents paint an alarmingly different picture.

This was a pedophile farm, said attorney Darrell Cochran, of Tacoma, who represents plaintiffs in all four cases, two of which were filed Tuesday.

The lawsuits each allege physical, sexual and emotional abuse by both staff and residents of the home, intentional understaffing with unqualified workers and financial fraud and negligence by staff and state agencies, including the state Department of Social and Health Services, which licensed the facility.

These individual defendants continued to support KVH despite clear evidence that it was a breeding ground for sexual abuse and sexually charged physical abuse, the lawsuit states.

Furthermore, Cochran said evidence gathered in the cases shows conspiracy rife with political corruption with the facility acting to conceal allegations of abuse while continuing to profit from state reimbursements for services.

Read the full article at...

08:12

Piperlongumine and analogues could be beneficial in breast cancer treatment. GreenMedInfo

PMID:  Eur J Med Chem. 2017 Jan 5 ;125:453-463. Epub 2016 Sep 16. PMID: 27689728 Abstract Title:  Piperlongumine (piplartine) and analogues: Antiproliferative microtubule-destabilising agents. Abstract:  Piperlongumine (piplartine, 1) is a small molecule alkaloid that is receiving intense interest due to its antiproliferative and anticancer activities. We investigated the effects of 1 on tubulin and microtubules. Using both an isolated tubulin assay, and a combination of sedimentation and western blotting, we demonstrated that 1 is a tubulin-destabilising agent. This result was confirmed by immunofluorescence and confocal microscopy, which showed that microtubules in MCF-7 breast cancer cells were depolymerized when treated with 1. We synthesised a number of analogues of 1 to explore structure-activity relationships. Compound 13 had the best cytotoxic profile of this series, showing potent effects in human breast carcinoma MCF-7 cells whilst being relatively non-toxic to non-tumorigenic MCF-10a cells. These compounds will be further developed as potential clinical candidates for the treatment of breast cancer.

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08:09

Public Health is At Risk: The Sobering Truth About Imported Chicken From China Youre Not Being Told AltHealthWorks.com

 

Unlabeled Chinese chicken imports to the U.S. may be on the rise thanks to a newly-reached trade deal. Photo via China Daily

 

08:01

Preventive potentials of piperlongumine and a Piper longum extract against stress responses and pain. GreenMedInfo

PMID:  J Tradit Complement Med. 2016 Oct ;6(4):413-423. Epub 2015 Dec 11. PMID: 27774429 Abstract Title:  Preventive potentials of piperlongumine and a Piper longum extract against stress responses and pain. Abstract:  AIM: To compare stress resistance increasing and analgesic activities of piperlongumine and a methanolic Piper longum fruit extract (PLE).METHODS: Efficacies of a single and repeated daily oral doses (1-256 mg/kg/day) of PLE, piperlongumine, and 50 mg/kg/day doxycycline against foot shock stress triggered alteration in body weights and core temperatures, and of their 11 daily doses on antidepressants like activity in tail suspension test and on pentobarbital induced sedation in male mice were compared. In another experiment, analgesic activities of single and repeated daily 5 mg/kg oral doses of piperlongumine and PLE in mice hot plate test and in acetic acid induced writing tests were compared with those of aspirin and doxycycline.RESULTS: After their single oral doses no effects of piperlongumine or PLE or doxycycline were observed in the footshock stress induced hyperthermia test or in hot plate test. However, significant effects of piperlongumine and PLE in both the tests were observed after their 5 or more daily doses. Both of them also dose dependently suppressed daily handling and repetitive testing triggered alterations in body weights and core temperatures. Their doxycycline like antidepressant activity in tail suspension test and aspirin like analgesic effects in acetic acid writhing test were observed after their 11 daily 5 mg/kg oral dose.CONCLUSION: Piperlongumine is another bioactive secondary metabolite of P. longum and other plants of piper species with stress response suppressing, analgesic, and anti-inflammatory activities. Its bactericidal activities can also contribute to its therapeutically interesting bio-activity profile.

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07:52

Piperlongumine could be used to overcome bortezomib resistance in multiple myeloma. GreenMedInfo

PMID:  Oncotarget. 2016 Nov 8 ;7(45):73497-73508. PMID: 27634873 Abstract Title:  Piperlongumine induces apoptosis and reduces bortezomib resistance by inhibiting STAT3 in multiple myeloma cells. Abstract:  Effective new therapies are urgently needed for the treatment of multiple myeloma (MM), an incurable hematological malignancy. In this study, we evaluated the effects of piperlongumine on MM cell proliferation both in vivo and in vitro. Piperlongumine inhibited the proliferation of MM cells by inducing cell apoptosis and blocking osteoclastogenesis. Notably, piperlongumine also reduced bortezomib resistance in MM cells. In a disseminated MM mouse model, piperlongumine prolonged the survival of tumor-bearing mice without causing any obvious toxicity. Mechanistically, piperlongumine inhibited the STAT3 signal pathway in MM cells by binding directly to the STAT3 Cys712 residue. These findings suggest that the clinical use of piperlongumine to overcome bortezomib resistance in MM should be evaluated.

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07:35

Piperlongumine could be useful for the treatment for multiple sclerosis. GreenMedInfo

PMID:  Free Radic Biol Med. 2017 Feb ;103:133-145. Epub 2016 Dec 21. PMID: 28011150 Abstract Title:  Piperlongumine attenuates experimental autoimmune encephalomyelitis through inhibition of NF-kappaB activity. Abstract:  Multiple sclerosis (MS) is a chronic, autoimmune and neurodegenerative disease in which demyelination sporadically and repeatedly occurs in the central nervous system (CNS). The activity of nuclear factor kappa B (NF-B), a family of transcription factors, was increased in the cerebrospinal fluid (CSF) and/or the serum and brain and/or spinal cord of MS patients than in a healthy donors. In our study, we investigated whether piperlongumine (PL), which is known to have inhibitory effect on activity of NF-B, can alleviate an experimental autoimmune encephalomyelitis (EAE). The mice were immunized with myelin oligodendrocyte glycoprotein 35-55 (MOG35-55), and then we injected PL (1.5mg/kg/day or 3.0mg/kg/day) into the mice intraperitoneally on every second day from days 2 to 28. For in vitro study, we treated PL (0.5, 1 and 2.5M) to RAW 264.7 and Jurkat cells with each stimulator. We observed that the paralytic severity and neuropathology of EAE in PL-treated group were decreased compared with the EAE group. PL showed a suppressed effect on demyelination, immune cells infiltration, astrocytes/microglials activation, level of inflammatory cytokines and proteins as well as NF-B activity. Production of inflammatory cytokines and proteins as well as translocation of NF-B into nucleus by treatment stimulators in RAW 264.7 and Jurkat cells were reduced by PL. Moreover, treatment of NF-B inhibitor further inhibited production of inflammatory cytokines and proteins. These results suggest that PL can mitigate MOG-induced EAE symptoms and activation of macrophages and T cells by inhibiting NF-B signaling. Therefore, PL could be useful for the treatment for MS.

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07:35

Pedophile Farm Alleged in Lawsuit Against Washington Foster Care Home for Boys Medical Kidnap

Centralias Kiwanis Vocational Home News Stories

Chronicle headlines in the 1980s and 1990s alluded to trouble at the Kiwanis Vocational Home, but nothing as explosive as claims included in a number of lawsuits filed in relation to alleged abuse, negligence and fraud at the facility, which closed in 1994. Image Source.

By Natalie Johnson
The Chronicle

Excerpts:

Centralias Kiwanis Vocational Home, open from 1979 to 1994, was intended to be a safe place for wayward boys, a state-licensed foster home where 11- to 17-year-olds could get an education and job skills in a family atmosphere, according to a 1986 Chronicle article.

However, four lawsuits from former residents paint an alarmingly different picture.

This was a pedophile farm, said attorney Darrell Cochran, of Tacoma, who represents plaintiffs in all four cases, two of which were filed Tuesday.

The lawsuits each allege physical, sexual and emotional abuse by both staff and residents of the home, intentional understaffing with unqualified workers and financial fraud and negligence by staff and state agencies, including the state Department of Social and Health Services, which licensed the facility.

These individual defendants continued to support KVH despite clear evidence that it was a breeding ground for sexual abuse and sexually charged physical abuse, the lawsuit states.

Furthermore, Cochran said evidence gathered in the cases shows conspiracy rife with political corruption with the facility acting to conceal allegations of abuse while continuing to profit from state reimbursements for services.

Read the full article at...

07:22

Marijuana treatment of PTSD and chronic pain probably does not work Skeptical Raptor

The Skeptical Raptor, stalking pseudoscience in the internet jungle.

Ive written extensively about marijuana treatment for various diseases. For example, using it to prevent or treat cancer? No clinical evidence support its use. In fact, a large review of published science on medical marijuana showed little evidence of it having a clinical benefit except for just a few conditions, one of which was chronic pain.

Skeptical Raptor

07:03

Protein regulator of cytokinesis 1 is a novel downstream target of piperlongumine in gastric cancer. GreenMedInfo

PMID:  J Cell Mol Med. 2017 Jul ;21(7):1329-1341. Epub 2017 Feb 12. PMID: 28190297 Abstract Title:  Elevated PRC1 in gastric carcinoma exerts oncogenic function and is targeted by piperlongumine in a p53-dependent manner. Abstract:  Gastric carcinoma is one of the most common malignancies worldwide and the second most frequent cause of cancer-related death in China. Protein regulator of cytokinesis 1 (PRC1) is involved in cytokinesis and plays key roles in microtubule organization in eukaryotes. This study was aimed to analyse the expression and to investigate the functional role of PRC1 in gastric tumorigenesis. The expression of PRC1 was evaluated by qRT-PCR, Western blot and immunohistochemistry. The biological function of PRC1 was determined by CCK-8 proliferation assays, monolayer colony formation, xenografted nude mice and cell invasion assays by shRNA-mediated knockdown in AGS and HGC27 cells. The regulation of PRC1 expression by piperlongumine was also investigated using dual-luciferase reporter assay and ChIP-qPCR analysis. PRC1 was up-regulated in primary gastric cancers. Overexpression of PRC1 in gastric cancers was associated with poor disease-specific survival and overall survival. PRC1 knockdown in AGS and HGC27 cell lines suppressed proliferation, reduced monolayer colony formation, inhibited cell invasion and migration ability and induced cell-cycle arrest and apoptosis. Inhibition of PRC1 also suppressed tumour growth in vivo. We finally confirmed that PRC1 is a novel downstream target of piperlongumine in gastric cancer. Our findings supported the oncogenic role of PRC1 in gastric carcinogenesis. PRC1 might serve as a prognostic biomarker and potential therapeutic target for gastric carcinoma.

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06:52

Piperlongumine downregulates the expression of HER family in breast cancer cells. GreenMedInfo

PMID:  Biochem Biophys Res Commun. 2017 May 13 ;486(4):1083-1089. Epub 2017 Apr 1. PMID: 28377224 Abstract Title:  Piperlongumine downregulates the expression of HER family in breast cancer cells. Abstract:  HER family receptors are frequently deregulated in breast cancer and the deregulation of these receptors is associated with poor prognosis. Thus, these receptors are considered therapeutic targets. In the present study, we found that piperlongumine (PL) downregulates the expression of HER family receptors HER1, HER2, and HER3 in breast cancer cells. Downregulation of these receptors by PL is mediated through the generation of reactive oxygen species (ROS), as N-acetyl-cysteine blocks it. Interestingly, the HER2-overexpressing cell lines BT474 and SkBr3 are somewhat more sensitive to PL than the low HER2-expressing cell line MCF7. In addition, the overexpression of HER2 increases the sensitivity of MCF7 cells to PL. Collectively, our data indicate the therapeutic potential of PL in the treatment of breast cancer.

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06:34

Piperlongumine induces reactive oxygen species dependent downregulation of specificity protein transcription factors. GreenMedInfo

PMID:  Cancer Prev Res (Phila). 2017 Aug ;10(8):467-477. Epub 2017 Jul 3. PMID: 28673967 Abstract Title:  Piperlongumine Induces Reactive Oxygen Species (ROS)-Dependent Downregulation of Specificity Protein Transcription Factors. Abstract:  Piperlongumine is a natural product found in the plant species Piper longum, and this compound exhibits potent anticancer activity in multiple tumor types and has been characterized as an inducer of reactive oxygen species (ROS). Treatment of Panc1 and L3.6pL pancreatic, A549 lung, 786-O kidney, and SKBR3 breast cancer cell lines with 5 to 15mol/L piperlongumine inhibited cell proliferation and induced apoptosis and ROS, and these responses were attenuated after cotreatment with the antioxidant glutathione. Piperlongumine also downregulated expression of Sp1, Sp3, Sp4, and several pro-oncogenic Sp-regulated genes, including cyclin D1,survivin, cMyc, EGFR and hepatocyte growth factor receptor (cMet), and these responses were also attenuated after cotreatment with glutathione. Mechanistic studies in Panc1 cells showed that piperlongumine-induced ROS decreased expression of cMyc via an epigenetic pathway, and this resulted in downregulation of cMyc-regulated miRNAs miR-27a, miR-20a, and miR-17 and induction of the transcriptional repressors ZBTB10 and ZBTB4. These repressors target GC-rich Sp-binding sites to decrease transactivation. This pathway observed for piperlongumine in Panc1 cells has previously been reported for other ROS-inducing anticancer agents and shows that an important underlying mechanism of action of piperlongumine is due to downregulation of Sp1, Sp3, Sp4, and pro-oncogenic Sp-regulated genes. Cancer Prev Res; 10(8); 467-77. 2017 AACR.

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06:15

Piperlongumine inhibits TGF--induced epithelial-to-mesenchymal transition GreenMedInfo

PMID:  Eur J Pharmacol. 2017 Jul 19. Epub 2017 Jul 19. PMID: 28734931 Abstract Title:  Piperlongumine inhibits TGF--induced epithelial-to-mesenchymal transition by modulating the expression of E-cadherin, Snail1, and Twist1. Abstract:  Cancer is a life-threatening disease, and the occurrence of metastasis, which increases the lethality of primary tumors, is increasing. The epithelial-to-mesenchymal transition (EMT) is a biological process by which epithelial cells lose cell-cell adhesion properties and acquire mesenchymal properties, including motility and invasiveness. EMT is considered an early stage of metastasis; therefore, inhibiting EMT may be an effective anticancer therapy. In the present study, the antimetastatic effect of piperlongumine (PL) was assessed in human cancer cells. PL is a single component isolated from long pepper (Piper longum) and it has been studied for its antibacterial, antiangiogenic, and antidiabetic activities. Migration assays (wound healing assay) and transwell invasion assays showed that PL inhibited the migration and invasion of cancer cells. Western blotting and immunofluorescence imaging showed that TGF- upregulated the transcription factors Snail1 and Twist1 and downregulated E-cadherin, a marker of epithelial cells, inducing EMT. PL might inhibit TGF--induced EMT by downregulating Snail1 and Twist1 and upregulating E-cadherin in cancer cells. In summary, PL might inhibit TGF--induced EMT, suggesting that it is a promising anticancer agent.

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05:41

Piperlongumine B and analogs are promising and selective inhibitors for acetylcholinesterase. GreenMedInfo

PMID:  Eur J Med Chem. 2017 Aug 2 ;139:222-231. Epub 2017 Aug 2. PMID: 28802122 Abstract Title:  Piperlongumine B and analogs are promising and selective inhibitors for acetylcholinesterase. Abstract:  Piperlongumine B (19), an alkaloid previously isolated from long pepper (Piper longum) has been synthesized for the first time in a short sequence and in good yield together with 19 analogs. Screening of these compounds in Ellman's assays showed several of them to be good inhibitors of acetylcholinesterase while being less active for butyrylcholinesterase. Activity of the compounds increased with the ring size of the heterocycle, and a maximum of activity was observed for an analog holding 12 methylene groups in the aliphatic side chain. These compounds may be regarded as promising candidates for the development of efficient inhibitors of acetylcholinesterase being useful for the treatment of Alzheimer's disease.

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05:25

Parthenolide or piperlongumine that induce almost complete glutathione depletion and severe cell death in AML cells. GreenMedInfo

PMID:  J Biol Chem. 2013 Nov 22 ;288(47):33542-58. Epub 2013 Oct 2. PMID: 24089526 Abstract Title:  Targeting aberrant glutathione metabolism to eradicate human acute myelogenous leukemia cells. Abstract:  The development of strategies to eradicate primary human acute myelogenous leukemia (AML) cells is a major challenge to the leukemia research field. In particular, primitive leukemia cells, often termed leukemia stem cells, are typically refractory to many forms of therapy. To investigate improved strategies for targeting of human AML cells we compared the molecular mechanisms regulating oxidative state in primitive (CD34(+)) leukemic versus normal specimens. Our data indicate that CD34(+) AML cells have elevated expression of multiple glutathione pathway regulatory proteins, presumably as a mechanism to compensate for increased oxidative stress in leukemic cells. Consistent with this observation, CD34(+) AML cells have lower levels of reduced glutathione and increased levels of oxidized glutathione compared with normal CD34(+) cells. These findings led us to hypothesize that AML cells will be hypersensitive to inhibition of glutathione metabolism. To test this premise, we identified compounds such as parthenolide (PTL) or piperlongumine that induce almost complete glutathione depletion and severe cell death in CD34(+) AML cells. Importantly, these compounds only induce limited and transient glutathione depletion as well as significantly less toxicity in normal CD34(+) cells. We further determined that PTL perturbs glutathione homeostasis by a multifactorial mechanism, which includes inhibiting key glutathione metabolic enzymes (GCLC and GPX1), as well as direct depletion of glutathione. These findings demonstrate that primitive leukemia cells are uniquely sensitive to agents that target aberrant glutathione metabolism, an intrinsic property of primary human AML cells.

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05:12

Why Steve Jobs Didnt Let His Kids Use iPads (and Why You Shouldnt Either) Healthy Holistic Living

Parenting is facing its biggest challenge to date technology. Children used to go to their parents for everything from questions that needed answering, to comfort, to simple entertainment. Any parent (and even grandparent) can attest and relate to the apparent reality of iPhone Facetime versus real, eye-to-eye face time....

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The post Why Steve Jobs Didnt Let His Kids Use iPads (and Why You Shouldnt Either) appeared first on Healthy Holistic Living.

05:08

Man Wants to Know Why He Has to Pay for Solar Energy He Generates Himself Healthy Holistic Living

Our planet has provided us with so much, that we sometimes forget that it cant last forever. We take more than we give, and we are beginning to see the consequences right before our eyes. Thankfully, many are taking steps to try and reduce their carbon footprint. It can be...

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The post Man Wants to Know Why He Has to Pay for Solar Energy He Generates Himself appeared first on Healthy Holistic Living.

04:57

How to Stop Sugar Cravings for Good Healthy Holistic Living

This article is shared with permission from our friends at Be Well, by Dr. Frank Lipman. Have you ever noticed how when youre short on sleep, stressed-out, or exhausted, you tend to crave carbs? Especially the crappy, nutrient-free variety that, under normal circumstances, you could easily resist? Turns out that when...

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The post How to Stop Sugar Cravings for Good appeared first on Healthy Holistic Living.

04:19

The Great Salt-Sodium Hoax: Eating More Salt Might Actually Save Your Life Health Impact News

different types of salt (pink, sea, black, and with spices)

by Paul Fassa
Health Impact News

Around 2012, and immediately before and after, Health Impact News featured some seriously thought provoking attacks on reigning dogmatic nutritional salt myths. The reports were from several experts such as Dr. Joseph Mercola, Dr. David Brownstein, author of Salt Your Way to Health, and the satirical approach of Scottish M.D. Malcolm Kendrick.

My favorite Health Impact News article on the matter was by Dr. Kendrick, who explained that the low sodium diets seem to forget that salt is composed of two molecules, salt (Na) and chloride (Cl). The chemical formula for salt is NaCl, or sodium chloride, which is salt.

Then he flashed a study that warned low serum chlorine was more dangerous than previously considered, concluding:

Low, not high Serum Cl- (<100 mEq/L), is associated with greater mortality risk independent of obvious confounders. Further studies are needed to elucidate the relation between Cl- and risk. (Study abstract)

Despite the growing evidence that low sodium causes more harm than high sodium, and using unprocessed salt has more benefits than commercial processed table salt, the mostly unsubstantiated belief that salt needs to be demonized persists. The revelations of good science are continually crushed by the pressures of public health policy.

Or as Dr. Kendrick states,

the salt hypothesis is rather like a monster from a 1950s B movie. Every time you attack it with evidence it simply shrugs it off and grows even stronger.

To this day, the American Heart Association (AHA) considers consuming more than 2,300 milligrams (mg) of sodium a day, which is the FDA and other government guidelines limit, too much, while recommending an ideal limit of no more than 1,500 mg per day (around a half teaspoon) for most adults. (Source)

But in order to be at the 2,300 mg sodium limit, one needs to consume over 5,000 mg of salt as sodium chlo...

04:14

Brain Regeneration new research findings two

Brain Regeneration: Why It's Real & How To Do It
4Posted on: Thursday, August 24th 2017 at 6:30 am
Written By: Sayer Ji, Founder
This article is copyrighted by GreenMedInfo LLC, 2017

Have you ever wished you could regenerate those brain cells you sacrificed in college? Do you fear that your aging brain is in a perpetual state of decline? Medical science is being rewritten to show that we CAN improve the health of our brain, and that repairing damage is not only possible, its something anyone can do.
It is a commonly held misconception that the brain is beyond repair. Even the medical establishment has asserted that once we kill brain cells, they are gone forever. The fact is, the brain can repair itself, and as science is now proving, there is real benefit to simple practices that can help keep our brains sharp and elastic throughout our lifetime.
Rewriting the Story of Brain Health
The field of cognitive neuroscience is relatively new - only around one hundred years old - so its no surprise that we are constantly arriving at a newer and better understanding of how the neural circuitry of the human brain supports overall brain functioning.
For most of those one hundred years, it was believed that once damaged, the brain could not regenerate. Brain cells were finite, and any loss or injury would be suffered as a deficiency for the rest of that persons life. This created a false belief that the brain is essentially in a perpetual state of decline.
Although compelling evidence to the contrary was presented as early as 1960, medical dogma was (and is) slow to change. It wasnt until the 1980s when Fernando Nottebohms research at Rockefeller University clearly indicated that neurogenesis - production of new nerve cells, aka neurons - was taking place in the adult vertebrate brain.
The next big step in this scientific evolution would take more than thirty years. However, the pace of our understanding of how the brain is wired was about to take a quantum leap.
...

03:52

Parthenolide induces superoxide anion production by stimulating EGF receptor in MDA-MB-231 breast cancer cells. GreenMedInfo

PMID:  Int J Oncol. 2013 Dec ;43(6):1895-900. Epub 2013 Oct 14. PMID: 24126683 Abstract Title:  Parthenolide induces superoxide anion production by stimulating EGF receptor in MDA-MB-231 breast cancer cells. Abstract:  The sesquiterpene lactone parthenolide (PN) has recently attracted considerable attention because of its anti-microbial, anti-inflammatory and anticancer effects. However, the mechanism of its cytotoxic action on tumor cells remains scarcely defined. We recently provided evidence that the effect exerted by PN in MDA-MB-231 breast cancer cells was mediated by the production of reactive oxygen species (ROS). The present study shows that PN promoted the phosphorylation of EGF receptor (phospho-EGFR) at Tyr1173, an event which was observed already at 1 h of incubation with 25M PN and reached a peak at 8-16 h. This effect seemed to be a consequence of ROS production, because N-acetylcysteine (NAC), a powerful ROS scavenger, prevented the increment of phospho-EGFR levels. In addition fluorescence analyses performed using dihydroethidium demonstrated that PN stimulated the production of superoxide anion already at 2-3 h of incubation and the effect further increased prolonging the time of treatment, reaching a peak at 8-16 h. Superoxide anion production was markedly hampered by apocynin, a well known NADPH oxidase (NOX) inhibitor, suggesting that the effect was dependent on NOX activity. The finding that AG1478, an EGFR kinase inhibitor, substantially blocked both EGFR phosphorylation and superoxide anion production strongly suggested that phosphorylation of EGFR can be responsible for the activation of NOX with the consequent production of superoxide anion.Therefore, EGFR phosphorylation can exert a key role in the production of superoxide anion and ROS induced by PN in MDA-MB-231 cells.

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03:31

These data provide a theoretical basis for the clinical application of parthenolide in prevention and control of diabetic nephropathy. GreenMedInfo

PMID:  Nan Fang Yi Ke Da Xue Xue Bao. 2013 Oct ;33(10):1471-3. PMID: 24144749 Abstract Title:  [Effects of parthenolide on high glucose-induced cell proliferation, NF-B activation and MCP-1 expression in rat mesangial cells]. Abstract:  OBJECTIVE: To explore the effects of parthenolide (PTL) on high glucose-stimulated cell proliferation, NF-B activation and monocyte chemoattractant protein-1 (MCP-1) expression in rat mesangial cells (MCs).METHODS: MCs were cultured in normal glucose (5.6 mmol/L), high glucose (30 mmol/L), or high glucose with PTL. MTT assay was used to detect the cell proliferation. MCP-1 content in the supernatant was measured by ELISA, and the level of IB was detected by Western blotting to reflect NF-B activity. EMSA method was used to measure the activation of NF-B.RESULTS: MC proliferation, MCP-1 expression and NF-B activation were significantly enhanced and the expression of NF-B-binding protein IB was obviously reduced in cells cultured in high glucose. Application of PTL obviously abolished the effects of high glucose.CONCLUSION: PTL can suppress high glucose-stimulated NF-B activation and MCP-1 expression in rat MC. These data provide a theoretical basis for the clinical application of PTL in prevention and control of diabetic nephropathy.

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02:58

Parthenolide has potential therapeutic value for bone destructive disorders that are associated with increased bone resorption. GreenMedInfo

PMID:  BMB Rep. 2014 Aug ;47(8):451-6. PMID: 24314143 Abstract Title:  Parthenolide inhibits osteoclast differentiation and bone resorbing activity by down-regulation of NFATc1 induction and c-Fos stability, during RANKL-mediated osteoclastogenesis. Abstract:  Parthenolide, a natural product derived from Feverfew, prevents septic shock and inflammation. We aimed to identify the effects of parthenolide on the RANKL (receptor activator of NF-B ligand)-induced differentiation and bone resorbing activity of osteoclasts. In this study, parthenolide dose-dependently inhibited RANKL-mediated osteoclast differentiation in BMMs, without any evidence of cytotoxicity and the phosphorylation of p38, ERK, and IB, as well as IB degradation byRANKL treatment. Parthenolide suppressed the expression of NFATc1, OSCAR, TRAP, DC-STAMP, and cathepsin K in RANKL-treated BMMs. Furthermore, parthenolide down-regulated the stability of c-Fos protein, but could not suppress the expression of c-Fos. Overexpression of NFATc1 and c-Fos in BMMs reversed the inhibitory effect of parthenolide on RANKL-mediated osteoclast differentiation. Parthenolide also inhibited the bone resorbing activity of mature osteoclasts. Parthenolide inhibits the differentiation and bone-resolving activity of osteoclast by RANKL, suggesting its potential therapeutic value for bone destructive disorders associated with osteoclast-mediated bone resorption.

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02:44

Parthenolide induces apoptosis via TNFRSF10B and PMAIP1 pathways in human lung cancer cells. GreenMedInfo

PMID:  J Exp Clin Cancer Res. 2014 Jan 6 ;33:3. Epub 2014 Jan 6. PMID: 24387758 Abstract Title:  Parthenolide induces apoptosis via TNFRSF10B and PMAIP1 pathways in human lung cancer cells. Abstract:  BACKGROUND: Parthenolide (PTL) is a sesquiterpene lactone which can induce apoptosis in cancer cells and eradicate cancer stem cells such as leukemia stem cells, prostate tumor-initiating cells and so on. However, the mechanism remains largely unclear.METHODS: Lung cancer cells were treated with parthenolide and the cell lysates were prepared to detect the given proteins by Western Blot analysis, and the cell survival was assayed by SRB and MTT assay. Cell cycle was evaluated by DNA flow cytometry analysis. TNFRSF10B, PMAIP1, ATF4 and DDIT3 genes were knocked down by siRNA technique. Apoptosis was evaluated by using Annexin V-FITC/PI staining and flow cytometry analysis.RESULTS: Parthenolide (PTL) induces apoptosis and cell cycle arrest in human lung cancer cells. Moreover, PTL treatment in NSCLC cells increases expression of TNFRSF10B/DR5 and PMAIP1/NOXA. Silencing of TNFRSF10B or PMAIP1 or overexpression of CFLAR /c-FLIP (long form) could protect cells from PTL-induced apoptosis. Furthermore, PTL could increase the levels of endoplasmic reticulum stress hallmarks such as ERN1, HSPA5, p-EIF2A, ATF4 and DDIT3. Knockdown of ATF4 and DDIT3 abrogated PTL-induced apoptosis, which suggested that PTL induced apoptosis in NSCLC cells through activation of endoplasmic reticulum stress pathway. More importantly, we found that ATF4, DDIT3, TNFRSF10B and PMAIP1 were up-regulated more intensively, while CFLAR and MCL1 were down-regulated more dramatically by PTL in A549/shCDH1 cells than that in control cells, suggesting that PTL preferred to kill cancer stem cell-like cells by activating more intensive ER stress response in cancer stem cell-like cells.CONCLUSION: We showed that parthenolide not only triggered extrinsic apoptosis by up-regulating TNFRSF10B and down-regulating CFLAR, but also induced intrinsic apoptosis through increasing the expression of PMAIP1 and decreasing the level of MCL1 in NSCLC cells. In addition, parthenolide triggered stronger ER stress response in cancer stem cell-like cells which leads to its preference in apoptotic induction. In summary, PTL induces apoptosis in NSCLC cells by activating endoplasmic reticulum stress response.

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01:59

Albuquerque: Fluoride decision delayed until September Fluoride Action Network

Consumers will have to wait another month to find out whether the water utility will resume supplemental fluoridation of Albuquerques drinking water.

After hearing passionate appeals from 32 speakers both for and against fluoridation, members of the utility board on Wednesday voted against taking up a funding measure for immediate action, postponing the decision until Sept. 20.

The Albuquerque Bernalillo County Water Utility board considered a proposed $260,000 capital appropriation that would pay for equipment needed to allow the city to resume supplemental fluoridation, which ended in 2011.

Under board rules, a new measure cant be considered for immediate action unless five of the seven voting members agree. Only three members Bernalillo County Commissioners Wayne Johnson and Maggie Hart Stebbins and City Councilor Pat Davis voted to consider the measure Wednesday.

I dont believe we need to add more chemicals to our water, board member and City Councilor Trudy Jones said after the meeting. Thats medical engineering and thats something we dont have a right to do.

Hart Stebbins said she doubts waiting another month will change anyones mind about the issue.

It seems we have had a great deal of public comment over the last two years, particularly the last two months, she said. Hart Stebbins said she supports fluoridation because her district includes the International District, where many low income people lack dental care.

Fluoride is a mineral found naturally in Albuquerques water supply at levels of about 0.5 milligram per liter, according to a report prepared by the utility staff.

The city began supplemental fluoridation in 1972, but ended the practice in 2011 while federal officials considered new recommendations about fluoridation levels.

In 2015, the U.S. Public Health Service updated the federal drinking water standards, recommending that community water systems add fluoride to 0.7 milligrams per liter to prevent tooth decay. That recommendation prompted the utility to reconsider fluoridation during budget discussions earlier this year.

The boards anticlimactic vote Wednesday followed more than an hour of public comment that recalled many of the passionate opinions expressed at a June 21 meeting on the same issue. At least 70 people turned out Wednesday, and 32 signed up to speak.

Many who expressed support for fluoridation were dentists, dental hygienists and public health officials.

Community water fluoridation is not dangerous, but rotten teeth are, said H. Lynn

Carroll, public health director for the New Mexico Department of Health. Fluoridation has been shown to reduce the incidence of tooth decay by 25 percent in adults and children, he said.

I can assure you that community water fluoridation is safe and effective in preventing tooth decay, he said.

Opponents implicated fluoride in...

01:53

Anthocyanin rich foods or extracts may improve vascular health. GreenMedInfo

PMID:  Nutrients. 2017 Aug 20 ;9(8). Epub 2017 Aug 20. PMID: 28825651 Abstract Title:  The Effect of Anthocyanin-Rich Foods or Extracts on Vascular Function in Adults: A Systematic Review and Meta-Analysis of Randomised Controlled Trials. Abstract:  Anthocyanins are of interest due to their anti-oxidative and vasodilatory properties. Earlier reviews have shown that berries and other anthocyanin rich foods or extracts can improve vascular health, however the effect of anthocyanins on vascular function has not yet been reviewed. To address this gap in the literature, we conducted a systematic review and meta-analysis of randomised-controlled trials examining anthocyanin-rich foods or extracts on measures of vascular reactivity and/or stiffness in adults. Data from 24 studies were pooled as standardized mean difference (SMD) with 95% confidence intervals (CI). Anthocyanin consumption significantly improved flow-mediated dilation (FMD) following acute (SMD: 3.92%, 95% CI: 1.47, 6.38, p = 0.002; I = 91.8%) and chronic supplementation (SMD: 0.84%, 95% CI: 0.55, 1.12, p = 0.000; I = 62.5%). Pulse wave velocity was improved following acute supplementation only (SMD: -1.27 m/s, 95% CI: -1.96, -0.58, p = 0.000; I = 17.8%). These results support the findings of previous reviews that anthocyanin rich foods or extracts may indeed improve vascular health, particularly with respect to vascular reactivity measured by FMD. More research is required to determine the optimal dosage, and the long-term effects of consumption.

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01:48

Albuquerque: Vote to add fluoride to metros drinking water deferred Fluoride Action Network

A decision on a proposed plan that would once again add more fluoride to drinking water in Albuquerque will have to wait another month.

Wednesday night the Water Authoritys Governing Board voted 4-3 to defer a vote until September 20.

Fluoride operations were halted in 2011.

The issue was brought up again due to many board members believing it would benefit the publics health.

*Original article online at http://krqe.com/blog/2017/08/24/vote-to-add-fluoride-to-metros-drinking-water-deferred/

**Read a more detailed article on the vote in the Albuquerque Journal

 

01:41

First of Its Kind Survey Shows Vaccinated Children Get Sicker Blog RSS

One of the first formal assessments of longer-term health outcomes associated with the routine childhood vaccination program in the U.S. concludes that vaccinated children are more likely to be diagnosed with a chronic illness compared to unvaccinated children. The carefully designed cross-se...

01:40

Colombian aai has a more diverse polyphenolic profile and higher antioxidant activity than Brazilian aai. GreenMedInfo

PMID:  Food Chem. 2017 Feb 15 ;217:364-72. Epub 2016 Aug 29. PMID: 27664647 Abstract Title:  Polyphenolic composition and antioxidant activity of aai (Euterpe oleracea Mart.) from Colombia. Abstract:  Berries of Colombian Euterpe oleracea Mart. were analyzed for total phenolic content (TPC), anthocyanin (ACN) content, and antioxidant activity. Additionally, reversed-phase ultra-high performance liquid chromatography with photodiode array detection (RP-UHPLC-PDA) and heated electrospray ionization (HESI) multistage mass spectrometry (MS(n)) were used to determine the composition of phenolic compounds. Anthocyanin content was 0.570.39mg cyanidin-3-glucoside/g fresh weight (FW) and TPC was 6.072.17mg gallic acid equivalent (GAE)/g FW. The ABTS radical scavenging activity was 3.11.3mol Trolox equivalents (TE)/100g FW, whereas the DPPH value was 2693.1332.8mol TE/100g FW. Overall, results show that Colombian aai has a more diverse polyphenolic profile and higher antioxidant activity than Brazilian aai. This information could be useful in authentification procedures to differentiate Brazilian aai from Colombian aai when used as an alternative for the supply of this fruit during the time of shortage in Brazil.

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01:38

Indian Researchers Develops Teabags That Can Easily Remove Fluoride From Water Fluoride Action Network

01:31

Mom Claims Gardasil Vaccine Caused Daughters Death Vaxxter

The producers of the documentary, Vaxxed, recently interviewed a mother who claims her daughter committed suicide following the Gardasil vaccine. She details what she says was a life ruined due to the side effects allegedly...

The post Mom Claims Gardasil Vaccine Caused Daughters Death appeared first on Vaxxter.

01:30

Given wrong pathology slides, heart journal retracts paper Retraction Watch

A cardiology journal has retracted a paper after the authors were unable to provide correct pathology slides to replace the wrong ones submitted with the original manuscript. The paper is titled Aortic Valve Endocarditis and Coronary Angiography With Cerebral Embolic Protection, published on April 10, 2017 in The Journal of the American College of Cardiology: []

The post Given wrong pathology slides, heart journal retracts paper appeared first on Retraction Watch.

00:58

5 Reasons to Avoid SEITAN: Groovy Name for a Not So Groovy Ingredient The Healthy Home Economist

seitanIt never ceases to amaze me how manufacturers so brazenly play bait and switch with the food labels for their products. The latest ingredient game that educated consumers need to know about is seitan. This is especially true for those who may be unknowingly eating it in the form of sprouted breads such as the very popular brand Ezekiel.(...)
Read the rest of 5 Reasons to Avoid SEITAN: Groovy Name for a Not So Groovy Ingredient (1,083 words)

The post 5 Reasons to Avoid SEITAN: Groovy Name for a Not So Groovy Ingredient appeared first on The Healthy Home Economist.

00:20

All Things New Geoengineering Watch

zen-gardner_all-things-newSource: Born Outside The Box by Zen Gardner August 22, 2017 We might be surprised at just how much newness is coming our way but its here, ready or not. We can absorb it at our own speed and according to our own choice of course, but its here and available for energizing and awareness

00:13

Brain Regeneration: Why It's Real & How To Do It GreenMedInfo

Brain Regeneration: Why It's Real & How To Do It

Have you ever wished you could regenerate those brain cells you sacrificed in college? Do you fear that your aging brain is in a perpetual state of decline? Medical science is being rewritten to show that we CAN improve the health of our brain, and that repairing damage is not only possible, its something anyone can do.

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Thursday, 24 August

23:13

These 4 Amazing Benefits of Weightlifting Will Make You Want to Hit the Gym Today Natural Blaze

Weightlifting is a form of resistance training that uses different types of weights to increase the resistance against muscles. Unlike other sports, weightlifting focuses on the increasing the total amount of weight lifted through the snatch and clean jerk methods.1,2

While many of us are familiar with the benefits of exercising, the diverse range of benefits from weightlifting is often less discussed. When executed properly, continuous weightlifting can improve body physique, increase lean muscle mass, improve muscle strength and metabolism.

Based on the latest scientific research, we explore the diverse benefits of incorporating weightlifting into your training routine.

1. Improved Strength

Developing and improving power output and muscle strength are central to all weightlifting. Many of these movements rely on major muscle groups and stabilizing muscles, engaging the entire body and increasing strength throughout. With a focus on high velocity and high force movements, weightlifting is often considered superior for improving muscle strength, speed and power.2

Consistent weightlifting (over 2 years) has been noted in clinical trials to promote increase in testosterone levels. In the body, higher levels of testosterone can help increase an athletes power.2 Men can also take natural testosterone support supplements, such as DSN Code Black to increase these levels naturally. Check out the these surprising Dyna Storm Code Black reviews to understand how natural testosterone supplement can benefit all types of athletes.

...

22:30

What should a journal do when a scientist who committed misconduct submits a new paper? Retraction Watch

In December of last year, Chris Surridge found himself in a situation not uncommon to journal editors: A researcher who had been found to have committed misconduct had submitted a manuscript to the journal Surridge edits, Nature Plants. Retraction Watch readers may recall the name of the corresponding author, Patrice Dunoyer, who has had five papers []

The post What should a journal do when a scientist who committed misconduct submits a new paper? appeared first on Retraction Watch.

22:30

Statins beat supplements Science-Based Medicine

Red yeast rice supplements have poor quality control and there's no clear evidence they do anything beneficial. So why take it?

22:02

Psychedelic Medicine Is The Topic For Discussion PreventDisease.com

There is a growing body of evidence that some psychedelic drugs can be used to treat a variety of conditions. The potential, the people and the politics are all up for discussion.

21:53

House on Fire: A Mental Health Literacy Parable Mad In America

Below, I present a fictitious conversation between a client and therapist. This dialogue is inspired by discussions with my clients about their experiences in the mental health system. It highlights a number of problems that are described at the end of this post.

* * * * *

Therapist: How are you?
 
Client: My house is on fire!
 
Therapist: Im sorry to hear that. How are you feeling?
 
Client: Im terrified! My dog is trapped inside! All my possessions are burning! What am I going to do?
 
Therapist: I understand that youre upset. Whats going through your mind?
 
Client: I cant believe this is happening! It doesnt seem real. Its like Im dreaming or something.
 
Therapist: Do you also feel detached from yourself or your surroundings?
 
Client: Yeah, I feel like Im in a daze. You hear about this happening to people but never think it can happen to you.
 
Therapist: I understand. These are common symptoms of Acute Stress Disorder. Its a mental illness some people experience in response to a traumatic event.
 
Client: What do you mean mental illness? My house is on fire! My dog is trapped inside!
 
Therapist: Im not saying you have a mental illness, only that you might have one. Well have to wait two more days and see if your symptoms continue before we know for certain.
...

20:33

The Rise of Solitary Mad In America

From Dissent Magazine: In her recently published book 23/7: Pelican Bay Prison and the Rise of Long-Term Solitary Confinement, scholar and advocate Keramet Reiter discusses the political, institutional, and legal dynamics that led to the emergence of supermax prisons and long-term solitary confinement in the 1990s.

In settings like Pelican Bay, even the most resilient people tend to develop psychiatric symptoms: anxiety, paranoia, obsessive-compulsive rituals. Suicides are far more common in isolation units than in standard prison settings. In the 1890 U.S. Supreme Court case In re Medley, the majority reviewed the reasons why American prisons had abandoned solitary confinement half a century earlier: A considerable number of the prisoners fell, after even a short confinement, into a semi-fatuous condition . . . and others became violently insane. But those lessons were eventually forgotten.

Article

20:32

Division 32 APA Response to Racism & Violence in Charlottesville Mad In America

It is not hate that kills, as much as it is silence. That is why Division 32 of the American Psychological Association is encouraging all caring parties to likewise give voice to the values that stand for our very humanity, as Humanistic Psychology emphasizes. In response to hate speech and violence in Charlottesville, it is clear that the White House was unable to display spontaneous benevolent leadership and awareness of the role a president plays during national crises, stepping up in critical moments to set an ethical bar as well as legal ones. Yet, we neednt be surprised by this lag in conscience, a lack of emotional intelligence or well thought out advance planning born of a self-reflective exploration, because there is nothing on which to base such an expectation.

In the context of mindful approaches to psychotherapy, every moment of life conditions the moment that comes after it, or said another way, every moment is conditioned by the one that came before it, by the one that preceded it. We must act thoughtfully, mindfully, and compassionately because cause and effect are interdependent. Reactions are spontaneous, empathy and leadership organically arise to meet the moment, producing human, and humane, responses. Thus, what we saw in Charlottesville was the result of all the many, many moments that caused it, that came before, that preceded it.

Much of the country wept on Saturday, after Friday nights nightmarish torch-bearing foreshadowing of the next days flashbacks of racism, covered faces, hiding, and hatred. Many of us deeply inhaled on Friday night and found ourselves breathless, and remain so even today. The solution doesnt only lie where we already know there is a massive and huge problem, but in the danger of propagating a resigned silence. This ennui engulfs a person or group of persons in a vacuum of hopelessness. So hope rests fully in saying no to silence.

Heather Heyer was martyred in Charlottesville as result of racism. Her mother, Susan Bro described her daughters caring way: It was important to her to speak up for people she felt were not being heard, to speak up when injustices were happening.

As psychologists, we can similarly fight silence by becoming active voices on radio, television, the internet, local and national newspapers, blogs, website postings, and all the other platforms that exist for us, speaking and posting against bigotry and fear, spreading instead the words of inclusiveness, of love, of a radical one love for all others and for ourselves, that says a large no to hate, so large that love itself is felt as a backlash. We can withstand it, I know.

We need only continue the work of Rev. Dr. Martin Luther King, Jr., who will always be the arbiter of peaceful non-violence, a politics of love. Perhaps there is an intersection where politics and love can find a synchronicity in our time, modeling Dr. Kings dignity, civility, Godliness, and love, patie...

20:31

State Sees Boom in Number of Psychiatric Beds Mad In America

From The Boston Globe: Massachusetts has seen a significant increase in its number of psychiatric institutions since 2015. However, many psychiatric patients are still waiting for long periods in the emergency room because psychiatric institutions are understaffed.

Article

20:30

The Really Big Lies About Autism AGE OF AUTISM

By Anne Dachel For the last 10 years I've written often about "THE REALLY BIG LIE ABOUT AUTISM," which is that autism's always been here, we just called it something else. There is also another REALLY BIG LIE, and that...

15:30

Learn About Lovage Oil's Benefits Today Articles

What Is Lovage Oil?

Lovage oil is derived from both the roots and the leaves of the lovage plant (Levisticum officinale), a hardy, herbaceous perennial native to southern parts of Europe. Also called bastard celery in France and love parsley in England, lovage can grow up to 2 meters (6 feet) in height and resembles other umbellifers such as fennel, coriander and parsley, with large umbels of yellow-green flowers with seeds.

Depending on whether the roots are fresh or dried at the time of distillation, lovage oil can be yellow or dark brown in color. It's quite resinous and thick and has a strong floral aroma with a slight bitterness and a hint of celery.1 Conversely, the essential oil derived from the leaves is fairly thin and has a warm, fresh, sweet and spicy aroma.

Uses of Lovage Oil

Dried lovage leaves, seeds and roots are often used in vegetarian cuisine to infuse dishes with its signature savory flavor. It is usually added in soups, stocks, casseroles and salads as a meat substitute.

Its stems can be blanched and eaten as a candy or as a vegetable dipped in a special cheese sauce. Its dried seeds, on the other hand, are ground using mortar and pestle and sprinkled as a spice or seasoning on different kinds of breads and biscuits before baking. Lovage oil specifically extracted from the plant roots is widely used as a:

Fragrance component in soaps, cosmetic products and perfumes

Flavoring agent in liquors, nonalcoholic beverages and a variety of edibles

Composition of Lovage Oil

The essential oil from the leaves contains alpha-terpinyl acetate, cis- and trans-ligustilides, alpha-phellandrene and alpha-terpineol, while the root oil has butylidene, dihydrobutylidene, butylphthalide, ligustilide, senkyunolide and a number of other phthalides. Terpenoids and coumarins are also present.2 Lovage oil blends well with bay, galbanum, lavender, oakmoss, opopanax and rose essential oils.3

Benefits of Lovage Oil

The lovage plant is helpful in treating jaundice, chronic constipation, skin diseases and inflammation of the eyes. During the Middle Ages, this herb was very popular for healing rheumatism, malaria, sore throat and kidney stones.

Meanwhile, lovage oil has proven its efficacy in alleviating conditions of the muscles, joints and cir...

Loneliness More Hazardous to Your Health Than Obesity or Smoking Articles

By Dr. Mercola

Loneliness doesn't just affect your mind; it can also cause a number of health problems. For example, previous research1 shows feeling lonely can raise your blood pressure up to 14 points, with greater increases the longer loneliness persists. With that, the risk for heart disease and dementia also increases.2

More recently, researchers concluded social isolation and loneliness may have more severe consequences than obesity and smoking. Other recent research reveals the brain-related changes associated with feelings of loneliness start to take place after as little as 24 hours of isolation.3

Loneliness More Hazardous to Your Health Than Obesity

Negative emotions will invariably impact your physical well-being, and feeling lonely is no different. According to two meta analyses4,5 presented at the 2017 Annual Convention of the American Psychological Association, loneliness and social isolation which are similar but not identical pose greater threats to public health than obesity, raising your risk for premature death by as much as 50 percent. As reported by Medical News Today:6

"While loneliness and social isolation are often used interchangeably, there are notable differences between the two. Social isolation is defined as a lack of contact with other individuals, while loneliness is the feeling that one is emotionally disconnected from others.

In essence, a person can be in the presence of others and still feel lonely. According to a 2016 Harris Poll7 of more than 2,000 adults in the U.S., around 72 percent reported having felt lonely at some point in their lives. Of these adults, around 31 percent reported feeling lonely at least once a week."

The first analysis, which looked at 148 studies involving more than...

Insurance and Government Block the Transition to Mercury-Free Dentistry Articles

By Dr. Mercola

Polls and common sense tell us that when consumers learn dental amalgam is 50 percent mercury, they demand a safe alternative for their mouths and bodies. But millions of consumers who are ready to switch are trapped in a vicious system that insists on putting toxic mercury in their mouths. Two culprits freeze in this primitive pre-Civil War, tooth-damaging pollutant:

  1. Insurance companies, whose outdated policies push lower-income consumers into amalgam
  2. Government programs across the board, where factory-style dentistry by the Pentagon, the Indian Health Service, the Federal Bureau of Prisons, Medicaid and others block consumer access to 21st century oral health care

America and the world are fortunate to have Consumers for Dental Choice, headed by Charlie Brown, leading the charge against both pro-mercury insurance companies and pro-mercury government agencies with programs like Demand Your Choice. Throughout Mercury Awareness Week (August 20 through 27, 2017), I promise to match your gifts to this excellent nonprofit group dollar for dollar.

Why do I put my money where my mouth is matching up to $100,000? Because I have closely watched Consumers for Dental Choice, and I have witnessed one achievement followed by another, including:

  • Protecting rights of mercury-free dentists by defeating the notorious gag rule that prevented them from disclosing the truth about amalgams mercury
  • Gaining fact sheets for consumers in some states and cities
  • Winning at the Minamata Convention on Mercury so each ratifying nation (including the U.S. and Canada) must cut down amalgam use
  • Leading the campaign that succeeded in banning amalgam for children and pregnant and breast-feeding women in the huge 28-nation European Union
Donate to Consumers for Dental Choice

>>>>> Click Here <<<<<

 This dental consumer succinctly describes how insurance companies enrich the pro-mercury dentists at our expense: With my current employer insurance, mercury-free fillings aren't covered at all so I have paid out of pocket for all my mercury-free fillings. My current insurance covers amalgam completely.

How Insurance Policies and Government Programs Promote Mercury Fillings

In the U.S., many third-party payers including private insurance companies, federal government programs and state government programs no doubt pose...

12:44

The Disgusting Stuff in Flu Shots Vaxxter

By Dr. Sherri Tenpenny, DO, AOBNMM, ABIHM Its that time again: the hype has begun to push flu shots into the arms of everyone, from six-month-old infants to 96-year-old grandparents. Flu vaccines have been around...

The post The Disgusting Stuff in Flu Shots appeared first on Vaxxter.

12:38

Tumor bearing mice treated five times with photodynamic therapy using acai oil in nanoemulsion showed tumor volume reduction of 82%. GreenMedInfo

PMID:  J Photochem Photobiol B. 2017 Jan ;166:301-310. Epub 2016 Dec 9. PMID: 28024281 Abstract Title:  Photodynamic therapy mediated by acai oil (Euterpe oleracea Martius) in nanoemulsion: A potential treatment for melanoma. Abstract:  Melanoma is the most aggressive and lethal form of skin cancer, responsible for>80% of deaths. Standard treatments for late-stage melanoma usually present poor results, leading to life-threatening side effects and low overall survival. Thus, it is necessary to rethink treatment strategies and design new tools for the treatment of this disease. On that ground, we hereby report the use of acai oil in nanoemulsion (NanoA) as a novel photosensitizer for photodynamic therapy (PDT) used to treat melanoma in in vitro and in vivo experimental models. NIH/3T3 normal cells and B16F10 melanoma cell lines were treated with PDT and presented 85% cell death for melanoma cells, while maintaining high viability in normal cells. Flow cytometry indicated that cell death occurred by late apoptosis/necrosis. Tumor bearing C57BL/6 mice treated five times with PDT using acai oil in nanoemulsion showed tumor volume reduction of 82% in comparison to control/tumor group. Necrotic tissue per tumor area reached its highest value in PDT-treated mice, supporting PDT efficacy. Overall, acai oil in nanoemulsion was an effective photosensitizer, representing a promising source of new photosensitizing molecules for PDT treatment of melanoma, a tumor with an inherent tendency to be refractory for this type of therapy.

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12:29

Acai seed extract substantially reduced renal injury and prevented renal dysfunction. GreenMedInfo

PMID:  J Med Food. 2017 Jun 26. Epub 2017 Jun 26. PMID: 28650699 Abstract Title:  Effect of Euterpe oleracea Mart. Seeds Extract on Chronic Ischemic Renal Injury in Renovascular Hypertensive Rats. Abstract:  Previously, we have demonstrated that the seeds of Euterpe oleracia Mart. (aa) are rich in polyphenols with antihypertensive and antioxidant properties. This study evaluated the renal protective effects of the hydroalcoholic extract obtained from the seeds of aa (ASE) fruits in two-kidney, one-clip (2K1C) renovascular hypertension. Young male Wistar rats were used to obtain 2K1C and sham groups. Animals received ASE (200mg/(kgday) in drinking water) or vehicle for 40 days. We evaluated serum and urinary parameters, renal structural changes, and oxidative status. The increase in systolic blood pressure of the 2K1C group was accompanied by a decrease in left kidney volume and number of glomeruli, as well as an increase in glomerular volume and collagen deposition. ASE prevented the alterations of these parameters, except the reduced kidney volume. Serum levels of urea and creatinine and urinary protein excretion were increased in the 2K1C group and treatment with ASE improved all these functional parameters. The increased oxidative damage in the 2K1C group, assessed by lipid and protein oxidation, was prevented by ASE. The nitrite content and both expression and activity of antioxidant enzymes (superoxide dismutase-1, catalase, and glutathione peroxidase) were lower in the 2K1C group and restored by ASE. ASE substantially reduced renal injury and prevented renal dysfunction in 2K1C rats probably through its antihypertensive and antioxidant effects, providing a natural resource for treatment and prevention of renovascular hypertension-related abnormalities.

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12:16

An ethyl acetate fraction of aa seed reduced cell viability and caused necroptosis in the MCF-7 cells. GreenMedInfo

PMID:  J Pharm Pharmacol. 2017 Jun ;69(6):714-721. Epub 2017 Feb 17. PMID: 28211563 Abstract Title:  Cytotoxic analysis and chemical characterization of fractions of the hydroalcoholic extract of the Euterpe oleracea Mart. seed in the MCF-7 cell line. Abstract:  OBJECTIVES: To analyse the antineoplastic activity of fractions derived from the hydroalcoholic extract of Euterpe oleracea Mart. seed in the MCF-7 cell line and to identify the compounds responsible for the antineoplastic action.METHODS: Cells were treated with 10, 20, 40 and 60g/ml with the hexane, chloroform and ethyl acetate fraction (EAF) of the hydroalcoholic extract of aa seed, for 24 and 48 h. After treatment, cell viability was measured using MTT assay and cell death was assessed using the Annexin-Pi assay. The most cytotoxic fraction under study was analysedby mass spectrometry using an electrospray ionization source and a cyclotron analyser coupled to a Fourier transform. Data were analysed statistically by analysis of variance (ANOVA) or by Student's t-test, where appropriate.KEY FINDINGS: All fractions caused significant reduction in the cell viability, but the EAF was the most cytotoxic (P

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12:01

Acai berry water extracts might have potential as a wound healing agents. GreenMedInfo

PMID:  Toxicol Res. 2017 Apr ;33(2):149-156. Epub 2017 Apr 15. PMID: 28503264 Abstract Title:  Skin Wound Healing Effects and Action Mechanism of Acai Berry Water Extracts. Abstract:  The purpose of this study was to investigate the wound healing effect of acai berry water extracts (ABWE) and a possible underlying mechanism involved in its action using various in vitro and in vivo models. The wound healing effect of ABWE was evaluated by migration assay using HS68 fibroblast cells. In addition, its effect on mRNA expression of procollagen, fibronectin, and MMP-1 was determined. Moreover, the wound healing effect of ABWE was evaluated in in vivo wound models through macroscopic and microscopic observation. In addition, mRNA expression levels of wound related genes were determined. Results revealed that ABWE was not cytotoxic. It increased migration of HS68 fibroblast cells. ABWE increased mRNA expression levels of fibronectin but decreased the mRNA expression levels of MMP-1. ABWE also showed significantly potent wound healing effect in vivo based on macroscopic and histopathological observation and mRNA expression evaluation for wound related genes. Taken together, our results indicated that ABWE might have potential as a wound healing agent.

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11:50

Aa demonstrated protective effects against AOM/DSS-induced colon carcinogenesis. GreenMedInfo

PMID:  Gut Liver. 2017 Mar 15 ;11(2):243-252. PMID: 27965474 Abstract Title:  Aa Berries Inhibit Colon Tumorigenesis in Azoxymethane/Dextran Sulfate Sodium-Treated Mice. Abstract:  Background/Aims: The aim of this study was to investigate the protective effect of aa against azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced colorectal cancer development.Methods: The effect of aa on tumorigenesis was assessed by evaluating tumor incidence, multiplicity and invasiveness in the mouse colon. The levels of myeloperoxidase (MPO) and proinflammatory cytokines (tumor necrosis factor [TNF-], interleukin [IL]-1, and IL-6) were measured via enzyme-linked immunosorbent assay. Protein levels of cyclooxygenase 2 (COX-2), proliferating cell nuclear antigen (PCNA), B-cell lymphoma 2 (Bcl-2), Bcl-2-associated death promoter (Bad) and cleaved-caspase-3 were assessed by immunoblotting.Results: Administration of pellets containing 5% aa powder reduced the incidences of both colonic adenoma and cancer (adenoma, 23.1% vs 76.9%, respectively, p=0.006; cancer, 15.4% vs 76.9%, respectively, p=0.002). In the aa-treated mice, the MPO, TNF-, IL-1 and IL-6 levels in the colon were significantly down-regulated. Aa inhibitedPCNA and Bcl-2 expression and increased Bad and cleaved-caspase-3 expression. In vitro studies demonstrated that aa treatment reduced lipopolysaccharide-induced expression of TNF-, IL-1, IL-6 and COX-2 in murine macrophage RAW 264.7 cells.Conclusions: Aa demonstrated protective effects against AOM/DSS-induced colon carcinogenesis, which suggests that the intake of aa may be beneficial for the prevention of human colon cancer.

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10:47

Parthenolide could down-regulate the blood IL-1beta and TNF-alpha concentrations in model of knee osteoarthritis. GreenMedInfo

PMID:  Zhongguo Zhong Xi Yi Jie He Za Zhi. 2013 Oct ;33(10):1382-4. PMID: 24432684 Abstract Title:  [Effect of parthenolide on serum expressions of interleukin-1beta and tumor necrosis factor-alpha in rabbits with knee osteoarthritis]. Abstract:  OBJECTIVE: To observe the therapeutic effect of parthenolide (PTL) on rabbit knee arthritis (KOA) and its effects on serum expression of interleukin-1beta (IL-1beta) and contents of tumor necrosis factor-alpha (TNF-alpha).METHODS: Eight rabbits were randomly selected from 40 healthy pure-bred New Zealand rabbits as the normal control group. The KOA model was established in the rest 32 rabbits by plaster cast fixation of the right hind limb extension position. After modeling they were randomly divided into 4 groups, i.e., the model control group, the high dose PTL group, the middle dose PTL group, and the low dose PTL group, 8 in each group. Serum contents of IL-1beta and TNF-alpha were detected using enzyme-linked immunosorbent assay.RESULTS: Compared with the model group, IL-1beta and TNF-alpha concentration decreased in the 3 PTL groups (P

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10:40

A review of the combined effect of parthenolide and various anti-cancer drugs. GreenMedInfo

PMID:  Mini Rev Med Chem. 2014 ;14(3):222-8. PMID: 24552263 Abstract Title:  Combined effect of parthenolide and various anti-cancer drugs or anticancer candidate substances on malignant cells in vitro and in vivo. Abstract:  Despite the enormous advances in the development of new drugs, the efficacy of current antitumor therapies is still quite limited, most likely because of the high degree of cancer heterogeneity and cell signaling complexity. As a single drug does not necessarily eradicate the cancer, the use of drug combinations has been proposed, and numerous studies have already been conducted to examine the efficacy of this strategy. In the last decade, parthenolide, a plant-derived sesquiterpene lactone, was extensively studied and its potential to inhibit cancer cell growth in vitro was well documented. More recently, antitumor effects exerted by parthenolide in combination with various drugs routinely used in cancer treatment have been investigated in several laboratories. In this article studies that are underway in an attempt to improve the anticancer efficacy of chemotherapies through combination strategies involving parthenolide are summarized.

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10:33

Parthenolide exerts inhibitory effects on angiogenesis through the downregulation of VEGF/VEGFRs in colorectal cancer. GreenMedInfo

PMID:  Int J Mol Med. 2014 May ;33(5):1261-7. Epub 2014 Feb 25. PMID: 24573421 Abstract Title:  Parthenolide exerts inhibitory effects on angiogenesis through the downregulation of VEGF/VEGFRs in colorectal cancer. Abstract:  Parthenolide (PT) is responsible for the bioactivities of feverfew (Tanacetum parthenium). Apart from its potent anti-inflammatory effects, this compound has been reported to induce apoptosis in various cancer cells. However, little is known about its role in the process of tumor angiogenesis. In the present study, we investigated the effects and potential mechanisms of action of PT on angiogenesis in human colorectal cancer (CRC). The anti-angiogenic effects of PT were evaluated in cultured human umbilical vein endothelial cells (HUVECs) and in the human CRC cell lines, HT-29, SW620 and HCT116. PT markedly inhibited vascular cell migration and capillary-like structure formation even at a dose which had not effects on cell viability. PT also suppressed the expression of angiogenic biomarker proteins [vascular endothelial growth factor (VEGF), VEGF receptor (VEGFR)1 and VEGFR2] in both the HUVECs and CRC cells. Additionally, PT effectively inhibited tumor neovascularization in a HT-29 xenograft model. These results indicate that PT suppresses angiogenesis by reducing the expression of VEGF and its receptors and may be a viable drug candidate in anti-angiogenesis therapies for human CRC.

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10:27

Suppression of AMPK or autophagy can potentially enhance the anti-cancer effect of parthenolide on breast cancer cells. GreenMedInfo

PMID:  J Cell Biochem. 2014 Aug ;115(8):1458-66. PMID: 24619908 Abstract Title:  Inhibition of AMPK/autophagy potentiates parthenolide-induced apoptosis in human breast cancer cells. Abstract:  Parthenolide is the main bioactive component in feverfew, a common used herbal medicine, and has been extensively studied in relation to its anti-cancer properties. However there have been very few in-depth studies of the activities of this compound at the molecular level. Here, we showed that parthenolide increased reactive oxygen species (ROS), induced cell death, activated AMPK and autophagy, and led to M phase cell cycle arrest in breast cancer cells. Removal of ROS inhibited all parthenolide-associated events, such as cell death, AMPK activation, autophagy induction, and cell cycle arrest. Blockade of autophagy relieved cell cycle arrest, whereas inhibition of AMPK activity significantly repressed the induction of both autophagy and cell cycle arrest. These observations clearly showed that parthenolide-driven ROS activated AMPK-autophagy pathway. Furthermore, inhibition of either AMPK or autophagy significantly potentiated parthenolide-induced apoptosis. Therefore, our results show that parthenolide activates both apoptosis pathway and AMPK-autophagy survival pathway through the generation of ROS, and that suppression of AMPK or autophagy can potentially enhance the anti-cancer effect of parthenolide on breast cancer cells.

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10:26

The Best Bed Bug Sprays to Kill Bed Bugs Fast Healthy and Natural World

Most people use a combination of bed bug sprays and bed bug powders to kill off bed bugs fast. Bed bugs are notoriously difficult to get rid of and finding the right spray to eliminate bed bugs is not always easy. Many natural residual bed bug sprays contain natural ingredients that have a proven repellent and insecticidal action against these infesting bugs. Some feel that they have to resort to commercial bed bug sprays to completely get rid of an infestation of bed bugs.

Contrary to popular belief, having bed bugs in your home is not a sign of a dirty home. Bed bugs are commonly found in clean hotel rooms, on public transportation, and movie theaters. They can easily be brought into the home in luggage, backpacks, or by purchasing used furniture. They can also travel between apartments in large complexes.

Although there are many ways to eliminate these tiny crawling bugs from your home, this article focuses on the best bed bug sprays to exterminate bed bugs effectively. You will also learn if natural bed bug insecticide sprays really work and what the precautions when using chemical bug sprays are.

What are Bed Bugs

The scientific name for bed bugs is Cimex lectularius. They are small creatures that grow up to 5 mm in size, or about the size of an apple seed. They have a flat oval-shaped body and will become significantly larger if they have fed. According to the United States Environmental Protection Agency, bed bugs start life as a tiny egg, then grow into a nymph before becoming an adult.1

According to the Department of Entomology at the University of Kentucky, bed bugs are more active at night when they come out to feed. They feed on the blood of humans and pets, however, in cooler temperatures, can go up to 6 months without feeding.2

The best places to use bed bug sprays are in the tiny crevices around bed frames, in seams of mattresses, box springs, and headboards where bed bugs like to hide. The University of Kentucky says that unless the first signs of a bed bug infestation arent dealt with quickly, bed bugs can migrate to other rooms and infest those.

What are the Signs of Bed Bug Infestation?

To use sprays effectively to eliminate all traces of bed bugs, you need to know the signs of a bed bug infestation. The first sign that you may have bedbugs are some itchy bite marks on your skin when you wake up in the morning. Dr. Carol DerSarkissian on WebMD says that the bites from bed bugs cause red itchy bumps on the skin.3

To check for signs of a bedbug infestation, Dr. DerSarkissian says that you may notice rusty-colored spots on bed linen and mattresses. These spots could be either blood stains or bedbug excrement. You may also find shed skins around...

10:11

A review of the role of Anethum graveolens in the management of diabetes. GreenMedInfo

PMID:  J Trop Med. 2016 ;2016:1098916. Epub 2016 Oct 18. PMID: 27829842 Abstract Title:  The Role of Anethum graveolens L. (Dill) in the Management of Diabetes. Abstract:  Aim. There is evidence that Anethum graveolens (AG) has been used for centuries in Asian traditional medicine, and its constituents have useful effects on the control and management of diabetes and cardiovascular disorders. AG has many useful effects, including hypolipidemic and hypoglycemic effects, and it has been reported to reduce the incidence of diabetic complications. It acts mainly by affecting antioxidant capacity and change in some genes in glucose and lipid pathways. The aim of the present paper was to summarize pharmacological effects of AG in the management of diabetes. Methods. To prepare this review, a pharmacological and phytochemical literature survey was performed using Scopus, PubMed, and Web of Science. Also, some historical and ethnopharmacological literature sources were used. Results. This review plans to provide readers with an assessment of the pharmacological effects of AG, especially in diabetes. Conclusion. The paper highlights the therapeutic effects of AG which would aid in supporting their safe use in the management of diabetes and cardiovascular diseases.

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09:34

Feverfew extracts represents a promising alternative for the treatment of leishmaniasis. GreenMedInfo

PMID:  Exp Parasitol. 2014 Aug ;143:18-23. Epub 2014 May 5. PMID: 24810433 Abstract Title:  In vitro and in vivo antileishmania activity of sesquiterpene lactone-rich dichloromethane fraction obtained from Tanacetum parthenium (L.) Schultz-Bip. Abstract:  The discovery of new treatments for neglected diseases, including leishmaniasis, is a substantial challenge for scientific research. Plant extracts have shown potential in the selective treatment of tropical diseases. The present study evaluated the in vitro and in vivo antileishmania effects of a sesquiterpene lactone-rich dichloromethane fraction (DF) obtained from the aerial parts of Tanacetum parthenium (L.) Schultz-Bip. In vitro studies of the DF indicated an IC50 of 2.400.76 g mL(-1) against the promastigote form and 1.760.25 g mL(-1) against the axenic amastigote form of Leishmania amazonensis. In vivo intramuscular treatment with DF decreased the growth and size of footpad lesions in mice. The DF also significantly decreased the parasite population compared with animals that were treated with the reference drug. Plasma malondialdehyde levels were increased slightly by the DF, attributable to its parthenolide-rich composition that causes cellular apoptosis, compared with the control group, demonstrating treatment efficacy without toxicity or genotoxicity. Because the isolation and purification of plant compounds are costly and time-consuming and generate low yields, extract fractions, such as the DF studied herein, represent a promising alternative for the treatment of leishmaniasis.

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09:26

Parthenolide lowers the viability of MDA-MB-231 breast cancer cells. GreenMedInfo

PMID:  Oncol Rep. 2014 Jul ;32(1):167-72. Epub 2014 May 23. PMID: 24859613 Abstract Title:  The oxygen radicals involved in the toxicity induced by parthenolide in MDA-MB-231 cells. Abstract:  It has been shown that the sesquiterpene lactone parthenolide lowers the viability of MDA-MB-231 breast cancer cells, in correlation with oxidative stress. The present report examined the different radical species produced during parthenolide treatment and their possible role in the toxicity caused by the drug. Time course experiments showed that in the first phase of treatment (0-8 h), and in particular in the first 3 h, parthenolide induced dichlorofluorescein (DCF) signal in a large percentage of cells, while dihydroethidium (DHE) signal was not stimulated. Since the effect on DCF signal was suppressed by apocynin and diphenyleneiodonium (DPI), two inhibitors of NADPH oxidase (NOX), we suggest that parthenolide rapidly stimulated NOX activity with production of superoxide anion (O2-), which was converted by superoxide dismutase 1 (SOD1) into hydrogen peroxide (H2O2). In the second phase of treatment (8-16 h), parthenolide increased the number of positive cells to DHE signal. Since this event was not prevented by apocynin and DPI and was associated with positivity of cellsto MitoSox Red, a fluorochrome used to detect mitochondrial production of O2-, we suggest that parthenolide induced production of O2- at the mitochondrial level independently by NOX activity in the second phase of treatment. Finally, in this phase, most cells became positive to hydroxyphenyl fluorescein (HPF) signal, a fluorescent probe to detect highly reactive oxygen species (hROS), such as hydroxyl radical and peroxynitrite. Therefore, parthenolide between 8-16 h of treatment induced generation of O2- and hROS, in close correlation with a marked reduction in cell viability.

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09:19

Overexpression of RIP3 sensitizes MCF-7 and MDA-MB-231 breast cancer cells to parthenolide in vitro. GreenMedInfo

PMID:  Acta Pharmacol Sin. 2014 Jul ;35(7):929-36. Epub 2014 Jun 9. PMID: 24909514 Abstract Title:  RIP3 overexpression sensitizes human breast cancer cells to parthenolide in vitro via intracellular ROS accumulation. Abstract:  AIM: Receptor-interacting protein 3 (RIP3) is involved in tumor necrosis factor receptor signaling, and results in NF-B-mediated prosurvival signaling and programmed cell death. The aim of this study was to determine whether overexpression of the RIP3 gene could sensitize human breast cancer cells to parthenolide in vitro.METHODS: The expression of RIP3 mRNA in human breast cancer cell lines (MCF-7, MDA-MB-231, MDA-MB-435 and T47D) was detected using RT-PCR. Both MDA-MB-231 and MCF-7 cells were transfected with RIP3 expression or blank vectors via lentivirus. Cell viability was measured with MTT assay; intracellular ROS level and cell apoptosis were analyzed using flow cytometry.RESULTS: RIP3 mRNA expression was not detected in the four human breast cancer cell lines tested. However, the transfection induced higher levels of RIP3 protein in MCF-7 and MDA-MB-231 cells. Furthermore, overexpression of RIP3 decreased the IC50 values of parthenolide from 17.6 to 12.6mol/L in MCF-7 cells, and from 16.6 to 9.9 mol/L in MDA-MB-231 cells. Moreover, overexpression of RIP3 significantly increased parthenolide-induced apoptosis and ROS accumulation in MCF-7 and MDA-MB-231 cells. Pretreatment with N-acetyl-cysteine abrogated the increased sensitivity of RIP3-transfected MCF-7 and MDA-MB-231 cells to parthenolide.CONCLUSION: Overexpression of RIP3 sensitizes MCF-7 and MDA-MB-231 breast cancer cells to parthenolide in vitro via intracellular ROS accumulation.

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09:10

Parthenolide has antileishmanial effects In vitro. GreenMedInfo

PMID:  BMC Microbiol. 2014 Jun 10 ;14:152. Epub 2014 Jun 10. PMID: 24913205 Abstract Title:  Cell death in amastigote forms of Leishmania amazonensis induced by parthenolide. Abstract:  BACKGROUND: Leishmania amazonensis infection results in diverse clinical manifestations: cutaneous, mucocutaneous or visceral leishmaniasis. The arsenal of drugs available for treating Leishmania infections is limited. Therefore, new, effective, and less toxic leishmaniasis treatments are still needed. We verified cell death in amastigote forms of Leishmania amazonensis induced by the sesquiterpene lactone parthenolide.RESULTS: The tested compound was able to concentration-dependently affect axenic and intracellular amastigotes, with IC50 values of 1.3 M and 2.9 M, respectively after 72 h incubation. No genotoxic effects were observed in a micronucleus test in mice. Parthenolide induced morphological and ultrastructural changes in axenic amastigotes, including a loss of membrane integrity, swelling of the mitochondrion, cytoplasmic vacuoles, and intense exocytic activity in the region of the flagellar pocket. These results led us to investigate the occurrence of autophagic vacuoles with monodansylcadaverine and the integrity of the plasma membrane and mitochondrial membrane potential using flow cytometry. In all of the tests, parthenolide had positive results.CONCLUSIONS: Our results indicate that the antileishmanial action of parthenolide is associated with autophagic vacuole appearance, a reduction of fluidity, a loss of membrane integrity, and mitochondrial dysfunction. Considering the limited repertoire of existing antileishmanial compounds, the products derived from medicinal plants has been one the greatest advances to help develop new chemotherapeutic approaches.

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08:58

Parthenolide can exert anti-cancer effects by inducing cell apoptosis, activating autophagy and inhibiting cell proliferation. GreenMedInfo

PMID:  Asian Pac J Cancer Prev. 2014 ;15(12):4897-902. PMID: 24998560 Abstract Title:  Parthenolide-induced apoptosis, autophagy and suppression of proliferation in HepG2 cells. Abstract:  PURPOSE: To investigate the anticancer effects and underlying mechanisms of parthenolide on HepG2 human hepatocellular carcinoma cells.MATERIALS AND METHODS: Cell viability was assessed by MTT assay and cell apoptosis through DAPI, TUNEL staining and Western blotting. Monodansylcadaverin(MDC) and AO staining were used to detect cell autophagy. Cell proliferation was assessed by Ki67 immunofluorescence staining.RESULTS: Parthenolide induced growth inhibition in HepG2 cells. DAPI and TUNEL staining showed that parthenolide could increase the number of apoptotic nuclei, while reducing the expression of the anti-apoptotic protein Bcl-2 and elevating the expression of related proteins, like p53, Bax, cleaved caspase9 and cleaved caspase3. Parthenolide could induce autophagy in HepG2 cells and inhibited the expression of proliferation-related gene, Ki-67.CONCLUSIONS: Parthenolide can exert anti-cancer effects by inducing cell apoptosis, activating autophagy and inhibiting cell proliferation.

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08:50

Parthenolide induced growth inhibition and apoptosis in SiHa and MCF-7 cells. GreenMedInfo

PMID:  Pharm Biol. 2015 Jan ;53(1):104-9. Epub 2014 Oct 7. PMID: 25289524 Abstract Title:  Effect of parthenolide on growth and apoptosis regulatory genes of human cancer cell lines. Abstract:  CONTEXT: Parthenolide (a sesquiterpene lactone), a bioactive compound of Tanacetum parthenium (L.) Schultz Bip. (Asteraceae) herb, has been reported for antioxidant and anticancer activities.OBJECTIVE: The present study evaluated the effect of parthenolide on growth and apoptosis-regulatory genes of human cervical cancer (SiHa) and breast cancer (MCF-7) cell lines.MATERIALS AND METHODS: The cytotoxic activity of parthenolide (3.5-21M) was examined by MTT and LDH assays at 24 and 48h time intervals. Apoptotic activity was evaluated by expression analysis of multiple apoptosis-regulatory genes (i.e., p53, Bcl-2, Bax, caspase-3, -6, and -9) by reverse transcriptase-PCR and DNA fragmentation assay.RESULTS: Parthenolide inhibited the growth of SiHa and MCF-7 cell lines in a concentration-dependent manner at 24 and 48h time intervals (p

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08:42

SAHA and parthenolide has a synergistic effect in MDA-MB231 breast cancer cells. GreenMedInfo

PMID:  J Cell Physiol. 2015 Jun ;230(6):1276-89. PMID: 25370819 Abstract Title:  The synergistic effect of SAHA and parthenolide in MDA-MB231 breast cancer cells. Abstract:  The sesquiterpene lactone Parthenolide (PN) exerted a cytotoxic effect on MDA-MB231 cells, a triple-negative breast cancer (TNBC) cell line, but its effectiveness was scarce when employed at low doses. This represents an obstacle for a therapeutic utilization of PN. In order to overcome this difficulty we associated to PN the suberoylanilide hydroxamic acid (SAHA), an histone deacetylase inhibitor. Our results show that SAHA synergistically sensitized MDA-MB231 cells to the cytotoxic effect of PN. It is noteworthy that treatment with PN alone stimulated the survival pathway Akt/mTOR and the consequent nuclear translocation of Nrf2, while treatment with SAHA alone induced autophagic activity. However, when the cells were treated with SAHA/PN combination, SAHA suppressed PN effect on Akt/mTOR/Nrf2 pathway, while PN reduced the prosurvival autophagic activity of SAHA. In addition SAHA/PN combination induced GSH depletion, fall inm, release of cytochrome c, activation of caspase 3 and apoptosis. Finally we demonstrated that combined treatment maintained both hyperacetylation of histones H3 and H4 induced by SAHA and down-regulation of DNMT1 expression induced by PN. Inhibition of the DNA-binding activity of NF-kB, whichis determined by PN, was also observed after combined treatment. In conclusion, combination of PN to SAHA inhibits the cytoprotective responses induced by the single compounds, but does not alter the mechanisms leading to the cytotoxic effects. Taken together our results suggest that this combination could be a candidate for TNBC therapy.

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08:40

3 Simple Tricks to Effectively Optimize Turmeric for Maximum Bioavailability and Therapeutic Potency REALfarmacy.com

Source: 3 Simple Tricks to Effectively Optimize Turmeric for Maximum Bioavailability and Therapeutic Potency For more content like this visit REALfarmacy.com.

by PAUL FASSA Countless studies have demonstrated the medicinal benefits of this amazing herb which you can view here. Once you see the irrefutable proof that turmeric has a plethora of important health benefits  you will be eager to work it into your everyday diet. However, before you begin consuming this potent food medicine, you []

Source: 3 Simple Tricks to Effectively Optimize Turmeric for Maximum Bioavailability and Therapeutic Potency Learn more at REALfarmacy.com - Healthy News and Information.

08:32

Crystals in Urine: Causes, Home Treatments, and When To See a Doctor Healthy and Natural World

Crystals in urine are usually detected in a urine analysis. The only visible sign you may notice if you have urinary crystals is cloudy urine; however, some types of crystals in urine are not visible at all without a microscope. Your kidneys are needed to filter out waste products from the body and excess fluid, and these end up in urine. The kidneys are also responsible for regulating salt, urinary acid content, and other minerals in the urine. If you have kidney problems or your urine is too concentrated, urinalysis may show up that there are too many crystal substances in your pee.

Some simple reasons for the presence of urinary crystals are because of dehydration or the side effect of medicines. In these cases, drinking more water can help return your urine to its normal color and mineral content. Doctors will usually check for the presence of an abnormally high number of crystal-like substances in urine if they suspect you have a urinary tract infection, kidney stones, or some other kidney dysfunction. In more serious cases, its important to treat the underlying cause of urine that contains crystal-like substances.

This article looks at the various reasons why crystals can appear in a urine test and what you can do to treat the problem. First, lets look at what urinary crystals are and why having them in your urine can sometimes be a cause for concern.

What are Urinary Crystals?

The medical name for crystals in urine is crystalluria. Healthy urine may contain a few microscopic crystals and they may also form after you have given a urine sample. According to the American Association for Clinical Chemistry, the number and type of crystals that form in urine are connected with urine pH levels, the concentration of substances in urine, and urine temperature.1

Common Types of Crystals in Urine

When performing a urinalysis, doctors will look at the shape and structure of the urinary crystals to determine their type. Here are some of the common types of crystals that show up in urine and what they mean.

Uric acid crystals. Dr. Anne Poinier on WebMD says that uric acid is usually excreted when you pee. High levels of uric acid crystals can form kidney stones and cause inflammation in joints.2

Calcium oxalate crystals. These types of crystals can form if the blood contains high levels of calcium, oxalate (salts that occur in many plants and nuts), cystine (a type of amino acid), and too little fluid. According to the journal Calcified Tissue International, this type of urinary crystal can form into kidney stones.3

Cystine crystals. If the amino acid cystine leaks into your urine, it can show up as cystine crystals in a urinalysis. According to MedicineNet, this is a common inherit...

08:01

Chlorine in Swimming Pools Transforms Sunscreen into Cancer-Causing Toxic Chemical Right on Your Skin REALfarmacy.com

Source: Chlorine in Swimming Pools Transforms Sunscreen into Cancer-Causing Toxic Chemical Right on Your Skin For more content like this visit REALfarmacy.com.

If you think youre doing something good by slathering sunscreen on yourself and your kids before hitting the pool, you might want to think again. A new study places sunscreen firmly on the list of products that can do more harm than good as Russian scientists discover the destructive effects of chlorine in swimming pools []

Source: Chlorine in Swimming Pools Transforms Sunscreen into Cancer-Causing Toxic Chemical Right on Your Skin Learn more at REALfarmacy.com - Healthy News and Information.

07:57

29 States Allow Big Ag To Act As Seed Legislator? Natural Blaze

By Heather CallaghanEditor

States like Oregon, California, Texas, Montana and Iowa just simultaneously nuked the ability to have seed laws. Claws of Big Ag found puppeteering legislators.

With little to no fanfare at all, the states passed seed preemption laws that essentially shut down public discourse on seeds laws including the use of genetically engineered crops. There is consternation that the move was designed to block counties and cities from adopting their own rules on the use of seeds, including bans on GMOs since in many parts of the country those crucial decisions are only the states to make.

Kristina Hubbard, director of advocacy and communications at the Organic Seed Alliance stated:

This bill should be viewed for what it is a gag order on public debate.

This thinly disguised attack on local democracy can be easily traced to out-of-state, corporate interests that want to quash local autonomy.

Opponents say the sudden measures from states like Texas, Montana and Iowa, reek of Big Agri efforts and are in the same league as ag-gag laws that prevent anyone from photographing farms or right-to-farm laws that may just ...

07:53

Parthenolide could become a promising and stable drug with anti-inflammatory effects. GreenMedInfo

PMID:  Nat Prod Res. 2015 ;29(12):1092-101. Epub 2014 Nov 28. PMID: 25429885 Abstract Title:  Parthenolide could become a promising and stable drug with anti-inflammatory effects. Abstract:  The aim of this paper is to summarise the blocking effects of parthenolide on the cytokines and pathways that cause inflammation. Analyses of the chemical structure of parthenolide have tried to identify the active domains that play key roles in its anti-inflammatory activity. This review focuses on the anti-inflammatory actions of parthenolide in both in vitro and in vivo. These data will be helpful for future investigations into the effects of parthenolide on inflammation.

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07:49

Vaccines and SIDS new research findings two

New Decision in US Vaccine Court SIDS Case Is Significant

Posted on: Monday, August 21st 2017 at 8:00 am
Written By: Jefferey Jaxen

Not reported by any major media outlet was a recent July 10th decision by the Office of Special Masters of the U.S. Court of Federal Claims, known as the vaccine court, that sufficient evidence was put forth to rule that vaccination caused a child to die from Sudden Infant Death Syndrome (SIDS). 
In order to better understand this decision by the vaccine court, it helps to consider some additional points first. Vaccines are indeed not safe. Not only do the manufacturers vaccine inserts include a laundry list of severe adverse reactions but, since its inception in 1986, the vaccine court has also begrudgingly paid out nearly $4 billion in compensation for injuries and deaths deemed to be a direct result of vaccines. 
The National Childhood Vaccine Injury Act of 1986 established a National Vaccine Injury Compensation Program (NVICP) to compensate vaccine-related injuries and deaths. Congress initially designed the NVICP to supplement the state law civil tort system as a simple, fair, and efficient means for compensating vaccine-injured people. The program was meant to award compensation quickly and easily. Unfortunately, independent investigation and countless horror stories from individuals who have experienced the vaccine court process have demonstrated clearly that the program is deeply flawed and highly adversarial towards petitioners. There is no jury trial within the vaccine court as it consists of administrative processes, sometimes resting solely upon the bias of the appointed special master overseeing the case.

Within the vaccine court, petitioners seeking compensation for their childrens SIDS cases are often denied, dismissed, or settled through an informal resolution before an official decision is made. What makes the recent case of BOATMAN & CUPID v. SECRETAR...

07:43

Parthenolide may be useful as a potential alternative or adjunct therapy for inflammatory arthritis. GreenMedInfo

PMID:  Scand J Rheumatol. 2015 May ;44(3):182-91. Epub 2014 Dec 2. PMID: 25439190 Abstract Title:  Parthenolide inhibits pro-inflammatory cytokine production and exhibits protective effects on progression of collagen-induced arthritis in a rat model. Abstract:  OBJECTIVES: Progressive destruction of synovial joint cartilage and bone occurs in pathological conditions such as rheumatoid arthritis (RA) because of the overproduction of pro-inflammatory cytokines and activation of nuclear factor kappa B (NF-B). Through the screening of NF-B inhibitors by a luciferase reporter gene assay, we identified parthenolide (PAR) as the most potent NF-B inhibitor, among several PAR analogue compounds. This study was undertaken to determine whether PAR inhibits pro-inflammatory cytokine production, cartilage degradation, and inflammatory arthritis.METHOD: The mRNA levels of pro-inflammatory cytokines were examined by real-time polymerase chain reaction (PCR). Proteoglycan content and release were determined by measuring glycosaminoglycan (GAG) levels using the dimethylmethylene blue (DMMB) dye-binding assay. The potential role of PAR in treatment of arthritis was studied using a collagen-induced arthritis (CIA) model.RESULTS: We established that PAR, as a prototype compound, suppressed lipopolysaccharide (LPS)- and tumour necrosis factor (TNF)--induced increases in matrix metalloproteinase (MMP)-1, MMP-3, inducible nitric oxide synthase (iNOS), and interleukin (IL)-1 mRNA in chondrocytes. In addition, PAR prevented proteoglycan degradation triggered by pro-inflammatory cytokines. PAR treatment at the onset of CIA symptoms significantly reduced synovitis, inflammation, and pannus formation scores. Reduced synovial inflammation after PAR treatment was also reflected in significantly less bone erosion and cartilage damage.CONCLUSIONS: These data indicate a protective effect of PAR on the catabolic insults of pro-inflammatory cytokines on chondrocyte metabolism and GAG release in vitro and in CIA. PAR had anti-inflammatory and structure-modifying effects on experimental arthritis, suggesting that PAR may be useful as a potential alternative or adjunct therapy for inflammatory arthritis.

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07:35

Parthenolide induced autophagy and may lead to therapeutic strategy to enhance the efficacy of anticancer drugs. GreenMedInfo

PMID:  Biochem Biophys Res Commun. 2015 Jan 2 ;456(1):434-9. Epub 2014 Dec 4. PMID: 25482447 Abstract Title:  Parthenolide induces autophagy via the depletion of 4E-BP1. Abstract:  Parthenolide (PTL) is a sesquiterpene lactone isolated from feverfew and exhibits potent antitumor activity against various cancers. Many studies indicate that PTL treatment leads to apoptosis, however, the mechanism has not been defined. Here, we observed that cells underwent autophagy shortly after PTL treatment. Inhibition of autophagy by knocking out autophagy associated gene atg5 blocked PTL-induced apoptosis. Surprisingly, PTL decreased the level of translation initiation factor eIF4E binding protein 1 (4E-BP1) in correlation with autophagy. Ectopic expression or shRNA knockdown of 4E-BP1 further verified the effect of 4E-BP1 on PTL-induced autophagy. Meanwhile, PTL elevated the cellular reactive oxygen species (ROS) which located upstream of the depletion of 4E-BP1, and contributed to the consequent autophagy. This study revealed 4E-BP1 as a trigger for PTL-induced autophagy and may lead to therapeutic strategy to enhance the efficacy of anticancer drugs.

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07:26

Parthenolide enhances sensitivity of colorectal cancer cells to TRAIL. GreenMedInfo

PMID:  Int J Oncol. 2015 Mar ;46(3):1121-30. Epub 2014 Dec 12. PMID: 25502339 Abstract Title:  Parthenolide enhances sensitivity of colorectal cancer cells to TRAIL by inducing death receptor 5 and promotes TRAIL-induced apoptosis. Abstract:  Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising cancer therapeutic agent. Recombinant human TRAIL has been evaluated in clinical trials, however, various malignant tumors are resistant to TRAIL. Parthenolide (PT) has recently been demonstrated as a highly effective anticancer agent and has been suggested to be used for combination therapy with other anticancer agents. In this study, we investigate the molecular mechanisms by which PT sensitizes colorectal cancer (CRC) cells to TRAIL-induced apoptosis. HT-29 (TRAIL-resistant) and HCT116 (TRAIL-sensitive) cells were treated with PT and/or TRAIL. The results demonstrated that combined treatment induced apoptosis which was determined using MTT, cell cycle analysis, Annexin V assay and Hoechst 33258 staining. Interestingly, we confirmed that HCT116 cells have much higher death receptor (DR) 5 than HT-29 cells and PT upregulates DR5 protein level and surface expression in both cell lines. Apoptosis through the mitochondrial pathway was confirmed by detecting regulation of Bcl-2 family members, p53 cytochrome C release, and caspase cascades. These results suggest that PT sensitizes TRAIL-induced apoptosis via upregulation of DR5 and mitochondria-dependent pathway. Combination treatment using PT and TRAIL may offer an effective strategy to overcome TRAIL resistance of certain CRC cells.

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07:18

Parthenolide inhibits cancer stem-like side population of nasopharyngeal carcinoma cells. GreenMedInfo

PMID:  Theranostics. 2015 ;5(3):302-21. Epub 2015 Jan 1. PMID: 25553117 Abstract Title:  Parthenolide inhibits cancer stem-like side population of nasopharyngeal carcinoma cells via suppression of the NF-B/COX-2 pathway. Abstract:  Cancer stem cells play a central role in the pathogenesis of nasopharyngeal carcinoma and contribute to both disease initiation and relapse. In this study, cyclooxygenase-2 (COX-2) was found to regulate cancer stem-like side population cells of nasopharyngeal carcinoma cells and enhance cancer stem-like cells' characteristics such as higher colony formation efficiency and overexpression of stemness-associated genes. The regulatory effect of COX-2 on cancer stem-like characteristics may be mediated by ABCG2. COX-2 overexpression by a gain-of-function experiment increased the proportion of side population cells and their cancer stemness properties. The present study also demonstrated that in contrast to the classical chemotherapy drug 5-fluorouracil, which increased the proportion of side population cells and upregulated the expression of COX-2, parthenolide, a naturally occurring small molecule, preferentially targeted the side population cells of nasopharyngeal carcinoma cells and downregulated COX-2. Moreover, we found that the cancer stem-like cells' phenotype was suppressed by using COX-2 inhibitors NS-398 and CAY10404 or knocking down COX-2 with siRNA and shRNA. These findings suggest that COX-2 inhibition is the mechanism by which parthenolide induces cell death in the cancer stem-like cells of nasopharyngeal carcinoma. In addition, parthenolide exhibited an inhibitory effect on nuclear factor-kappa B (NF-B) nucler translocation by suppressing both the phosphorylation of IB kinase complex and IB degradation. Taken together, these results suggest that parthenolide may exert its cancer stem cell-targeted chemotherapy through the NF-B/COX-2 pathway.

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07:03

These data offer fundamental evidence supporting the potential use of parthenolide in periodontitis treatment. GreenMedInfo

PMID:  Evid Based Complement Alternat Med. 2014 ;2014:546097. Epub 2014 Dec 25. PMID: 25610476 Abstract Title:  Anti-inflammatory and antiosteoclastogenic activities of parthenolide on human periodontal ligament cells in vitro. Abstract:  Periodontitis is an inflammatory disease that causes osteolysis and tooth loss. It is known that the nuclear factor kappa B (NF-B) signalling pathway plays a key role in the progression of inflammation and osteoclastogenesis in periodontitis. Parthenolide (PTL), a sesquiterpene lactone extracted from the shoots of Tanacetum parthenium, has been shown to possess anti-inflammatory properties in various diseases. In the studyreported herein, we investigated the effects of PTL on the inflammatory and osteoclastogenic response of human periodontal ligament-derived cells (hPDLCs) and revealed the signalling pathways in this process. Our results showed that PTL decreased NF-B activation, I-B degradation, and ERK activation in hPDLCs. PTL significantly reduced the expression of inflammatory (IL-1, IL-6, and TNF-) and osteoclastogenic (RANKL, OPG, and M-CSF) genes in LPS-stimulated hPDLCs. In addition, PTL attenuated hPDLC-induced osteoclastogenic differentiation of macrophages (RAW264.7 cells), as well as reducing gene expression of osteoclast-related markers in RAW264.7 cells in an hPDLC-macrophage coculture model. Taken together, these results demonstrate the anti-inflammatory and antiosteoclastogenic activities of PTL in hPDLCs in vitro. These data offer fundamental evidence supporting the potential use of PTL in periodontitis treatment.

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06:49

Parthenolide is a strong apoptotic inducer that deserves the further investigations for potential chemotherapeutic agent of human oral cancers. GreenMedInfo

PMID:  Oral Oncol. 2015 Jun ;51(6):602-9. Epub 2015 Mar 26. PMID: 25817195 Abstract Title:  Induction of apoptosis by parthenolide in human oral cancer cell lines and tumor xenografts. Abstract:  OBJECTIVES: Parthenolide (PTL), a representative sesquiterpene lactone that is responsible for medicinal properties of the feverfew, is known to modulate diverse intracellular signaling pathways, thereby exerting the tumor growth-inhibitory effects. In this study, authors attempted to examine the pro-apoptotic effects and possible biochemical mechanisms of PTL in human oral cancer cell lines and tumor xenografts.MATERIAL AND METHODS: The apoptotic effects and related molecular mechanisms of PTL on oral cancer were evaluated using cell viability assay, MTS assay, DAPI staining, western blot analysis, reverse transcriptase-polymerase chain reaction, small interfering RNA transfection and nude mouse xenograft assay.RESULTS: PTL treatment increased the cleavage of caspase-3 and poly (ADP-ribose) polymerase (PARP), and the nuclear fragmentation in a concentration- or time-dependent manner. PTL treatment increased Bim protein expression by enhancing the Bim mRNA expression as well as stabilizing Bim protein level. PTL treatment also induced the translocation of cytosolic Bim into the mitochondria and, more importantly, PTL-induced apoptosis was significantly attenuated, when the Bim expression was knockdown by siRNA transfection. PTL treatment also induced death receptor 5 (DR5) protein expression and this event was closely correlated with an increase in the cleavage of caspase-8 and formation of truncation of Bid (t-Bid). Finally, PTL shrunk tumor size and volume resulting from apoptotic cell death by increasing Bim and DR5 whereas there were no abnormal histopathological findings in normal organs.CONCLUSION: This study proposes that PTL is a strong apoptotic inducer that deserves the further investigations for potential chemotherapeutic agent of human oral cancers.

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06:38

Parthenolide inhibits LPS-induced inflammatory cytokines. GreenMedInfo

PMID:  Acta Biochim Biophys Sin (Shanghai). 2015 May ;47(5):368-75. Epub 2015 Apr 4. PMID: 25841439 Abstract Title:  Parthenolide inhibits LPS-induced inflammatory cytokines through the toll-like receptor 4 signal pathway in THP-1 cells. Abstract:  Parthenolide (PTL) shows potent anti-inflammatory and anti-cancer activities. In the present study, the molecular mechanisms of PTL's activities were explored in lipopolysaccharide (LPS)-induced human leukemia monocytic THP-1 cells and human primary monocytes. The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium salt (MTS) assay was used to analyze the effect of PTL on THP-1 cell viability. Enzyme-linked immunosorbent assay was used to determine the effect of PTL on LPS-induced inflammatory cytokine secretion. Flow cytometry and quantitative real-time polymerase chain reaction were used to assess the effect of PTL on LPS-induced toll-like receptor 4 (TLR4) expression. Phosphorylation levels of signaling molecules were determined by western blot analysis. Results showed that PTL

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06:30

Parthenolide induced survivin inhibition, cell cycle arrest and triggered cell death in glioblastoma cells. GreenMedInfo

PMID:  Chin J Physiol. 2015 Apr 30 ;58(2):95-103. PMID: 25858470 Abstract Title:  Induction of survivin inhibition, G/M cell cycle arrest and autophagic on cell death in human malignant glioblastoma cells. Abstract:  Chemotherapy efficacy is limited by intrinsic and acquired resistance in glioblastoma (GBM); hence, novel tactics are crucial. Survivin has been demonstrated as a key resistant factor in GBM because of its function in inhibiting apoptosis, regulating autophagy, and in promoting G/M cell cycle transition. Parthenolide has been reported to be an effective antitumor agent in a variety of tumor cells and decreases survivin level in leukemia cells. But the effect of parthenolide on survivin and the cell death process in GBM is still unknown. The aim of this study was to examine whether parthenolide had the potential to inhibit cell proliferation in the GBM cell line U373. The parthenolide-induced effects in relation to survivin suppression and cell death were further investigated. Our results showed that parthenolide substantially inhibited cell viability with ICvalues of approximate 16 M. Treatment with parthenolide at the dose of 16 M led to considerable downregulation of survivin, G/M cell cycle arrest and Chk2 upregulation in cells. Parthenolide induced apoptosis in only a few cells and a slight increase in activated caspases 3 levels. By contrast, parthenolide induced a significant increase of intracellular autophagosomes and the expression of autophagy related proteins, including ULK1 and LC3 I/LC3 II, in the treated cells. These results suggested that parthenolide induced survivin inhibition, G/M cell cycle arrest, and triggered celldeath through autophagic cell death in the GBM cell line.

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06:13

Parthenolide inhibited cell proliferation, induced apoptosis and blocked cell cycle. GreenMedInfo

PMID:  J Huazhong Univ Sci Technolog Med Sci. 2015 Jun ;35(3):343-9. Epub 2015 Jun 14. PMID: 26072071 Abstract Title:  Inhibitory effects of parthenolide on the activity of NF-B in multiple myeloma via targeting TRAF6. Abstract:  This study examined the mechanism of the inhibitory effect of parthenolide (PTL) on the activity of NF-B in multiple myeloma (MM). Human multiple myeloma cell line RPMI 8226 cells were treated with or without different concentrations of PTL for various time periods, and then MTT assay was used to detect cell proliferation. Cell cycle and apoptosis were flow cytometrically detected. The level of protein ubiquitination was determined by using immunoprecipitation. Western blotting was employed to measure the level of total protein ubiquitination, the expression of IB- in cell plasma and the content of p65 in nucleus. The content of p65 in nucleus before and after PTL treatment was also examined with immunofluorescence. Exposure of RPMI 8226 cells to PTL attenuated the level of ubiquitinated Nemo, increased the expression of IB- and reduced the level of p65 in nucleus, finally leading to the decrease of the activity of NF-B. PTL inhibited cell proliferation, induced apoptosis andblocked cell cycle. Furthermore, the levels of ubiquitinated tumor necrosis factor receptor-associated factor 6 (TRAF6) and total proteins were decreased after PTL treatment. By using Autodock software package, we predicted that PTL could bind to TRAF6 directly and tightly. Taken together, our findings suggest that PTL inhibits the activation of NF-B signaling pathway via directly binding with TRAF6, thereby suppressing MM cell proliferation and inducing apoptosis.

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06:04

Parthenolide appears to attenuate the microbial product-mediated inflammatory skin diseases. GreenMedInfo

PMID:  Naunyn Schmiedebergs Arch Pharmacol. 2015 Sep ;388(9):921-30. Epub 2015 May 15. PMID: 25971793 Abstract Title:  Sesquiterpene lactone parthenolide attenuates production of inflammatory mediators by suppressing the Toll-like receptor-4-mediated activation of the Akt, mTOR, and NF-B pathways. Abstract:  Microbial product lipopolysaccharide has been shown to be involved in the pathogenesis of inflammatory skin diseases. Parthenolide present in extracts of the herb feverfew has demonstrated an anti-inflammatory effect. However, the effect of parthenolide on the Akt/mTOR and NF-B pathway activation-induced productions of inflammatory mediators in keratinocytes has not been studied. Using human keratinocytes, we investigated the effect of parthenolide on the inflammatory mediator production in relation to the Toll-like receptor-4-mediated-Akt/mTOR and NF-B pathways, which regulate the transcription genes involved in immune and inflammatory responses. Parthenolide, Akt inhibitor, Bay 11-7085, and N-acetylcysteine each attenuated the lipopolysaccharide-induced production of IL-1 and PGE2, increase in the levels of cyclooxygenase, formation of reactive oxygen species, increase in the levels of Toll-like receptor-4, and activation of the Akt/mTOR and NF-B in keratinocytes. The results show that parthenolide appears to attenuate the lipopolysaccharide-stimulated production of inflammatory mediators in keratinocytes by suppressing the Toll-like receptor-4-mediated activation of the Akt, mTOR, and NF-B pathways. The activation of signaling transduction pathways appear to be regulated by reactive oxygen species. Parthenolide appears to attenuate the microbial product-mediated inflammatory skin diseases.

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06:02

Iatrogenesis and Big Pharmas Lucrative Overvaccination Agendas Natural Blaze

By Gary G. Kohls, MD, guest to Natural Blaze

Ignaz Semmelweis, Galileo Galilei, Andrew Wakefield and the Semmelweis Reflex:

Iatrogenesis and Big Pharmas Lucrative Overvaccination Agendas

It is dangerous to be right in matters on which the established authorities are wrong.  Voltaire

If you offend the powers that be; if you offend the public health establishments; if you offend the pharmaceutical industry and threaten their bottom line (as we did by calling into question the safety of MMR [and later all the other vaccines that contain brain-toxic, mitochondrial-toxic and blood-brain barrier-toxic ingredients like aluminum and mercury ed note]) there is no price that you will not pay. You will be destroyed in order to protect their bottom line. Dr Andrew Wakefield

Anything that implies that immunizations are not the greatest medical advance in the history of public health is ignored or ridiculed. Can you imagine the economic and political import of discovering that immunizations are killing (or maiming) thousands of babies? Dr William C. Douglass, MD (Honored twice as Americas Doctor of the Year)

I was told by a network executive that during non-election years up to 70% of his news divisions revenues are from pharmaceutical corporations advertising. That executive told me that if one of his t...

05:54

Parthenolide directly or indirectly attenuates neuropathy symptoms and promotes M2 microglia/macrophages polarization. GreenMedInfo

PMID:  Neural Plast. 2015 ;2015:676473. Epub 2015 May 18. PMID: 26090236 Abstract Title:  Parthenolide Relieves Pain and Promotes M2 Microglia/Macrophage Polarization in Rat Model of Neuropathy. Abstract:  Neuropathic pain treatment remains a challenge because pathomechanism is not fully understood. It is believed that glial activation and increased spinal nociceptive factors are crucial for neuropathy. We investigated the effect of parthenolide (PTL) on the chronic constriction injury to the sciatic nerve (CCI)-induced neuropathy in rat. We analyzed spinal changes in glial markers and M1 and M2 polarization factors, as well as intracellular signaling pathways. PTL (5g; i.t.) was preemptively and then daily administered for 7 days after CCI. PTL attenuated the allodynia and hyperalgesia and increased the protein level of IBA1 (a microglial/macrophage marker) but did not change GFAP (an astrocyte marker) on day 7 after CCI. PTL reduced the protein level of M1 (IL-1, IL-18, and iNOS) and enhanced M2 (IL-10, TIMP1) factors. In addition, it downregulated the phosphorylated form of NF-B, p38MAPK, and ERK1/2 protein level and upregulated STAT3. In primary microglial cell culture we have shown that IL-1, IL-18, iNOS, IL-6, IL-10, and TIMP1 are of microglial origin. Summing up, PTL directly or indirectly attenuates neuropathy symptoms and promotes M2 microglia/macrophages polarization. We suggest that neuropathic pain therapies should be shifted from blanketed microglia/macrophage suppression toward maintenance of the balance between neuroprotective and neurotoxic microglia/macrophage phenotypes.

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05:36

BIOSCAN & BIOTONOMETER TO BE AHEAD OF TIME Lyra Nara Blog

Worldwide Unique, Affordable, Modern Bioenergy Diagnostic What does modern therapy have to provide to practitioners, patients and private users? Customized therapy options Fast, accurate therapy results No need for other tools or devices Cost-effectiveness to save your money Easytooperate & understand Perfect for multiple applications (health, nutrition, supplementation, medication, fitness, etc.) We introduce BIOSCAN SWA

05:30

Combination treatment with parthenolide and balsalazide may offer an effective strategy for the induction of apoptosis in HCT116 cells. GreenMedInfo

PMID:  Intest Res. 2015 Jul ;13(3):233-41. Epub 2015 Jun 9. PMID: 26130998 Abstract Title:  Balsalazide Potentiates Parthenolide-Mediated Inhibition of Nuclear Factor-B Signaling in HCT116 Human Colorectal Cancer Cells. Abstract:  BACKGROUND/AIMS: Balsalazide is an anti-inflammatory drug used in the treatment of inflammatory bowel disease. Balsalazide can reduce inflammatory responses via several mechanisms, including inhibition of nuclear factor-B (NF-B) activity. Parthenolide (PT) inhibits NF-B and exerts promising anticancer effects by promoting apoptosis. The present investigated the antitumor effects of balsalazide, combined with PT, on NF-B in a representative human colorectal carcinoma cell line, HCT116.METHODS: We counted cells and conducted annexin-V assays and cell cycle analysis to measure apoptotic cell death. Western blotting was used investigate the levels of proteins involved in apoptosis.RESULTS: PT and balsalazide produced synergistic anti-proliferative effects and induced apoptotic cell death. The combination of balsalazide and PT markedly suppressed nuclear translocation of the NF-B p65 subunit and the phosphorylation of inhibitor of NF-B. Moreover, PT and balsalazide dramatically enhanced NF-B p65 phosphorylation. Apoptosis, through the mitochondrial pathway, was confirmed by detecting effects on Bcl-2 family members, cytochrome c release, and activation of caspase-3 and -8.CONCLUSIONS: Combination treatment with PT and balsalazide may offer an effective strategy for the induction of apoptosis in HCT116 cells.

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05:17

Peter Doshi flu vaccine study misused by anti-vaxxers Skeptical Raptor

The Skeptical Raptor, stalking pseudoscience in the internet jungle.

Anti-vaccine activists constantly look for any science that seems to support their beliefs about the safety and effectiveness of vaccines. Lately, they have gravitated to the writings of Peter Doshi, who has made a career talking about vaccines. Although Doshi lacks the credentials in any area of science related to vaccines immunology, microbiology, virology,

Skeptical Raptor

05:13

Parthenolide induces apoptosis in colitis-associated colon cancer. GreenMedInfo

PMID:  Oncol Lett. 2015 May ;9(5):2135-2142. Epub 2015 Mar 6. PMID: 26137027 Abstract Title:  Parthenolide induces apoptosis in colitis-associated colon cancer, inhibiting NF-B signaling. Abstract:  Recently, the nuclear factor (NF)-B inhibitor parthenolide (PT) was identified as a promising anticancer agent for the promotion of cancer cell apoptosis. Additionally, our previous study demonstrated that PT administration suppresses tumor growth in a xenograft model of colorectal cancer cells via regulation of the B-cell lymphoma-2 (Bcl-2) family. However, the role of PT in the development of colitis-associated colon cancer (CAC) is poorly understood. Therefore, the aim of the present study was to investigate the effects of PT administration on CAC using a murine model. Azoxymethane (AOM) and dextran sulfate sodium (DSS) were administered to induce experimental CAC in the following three groups of treated mice: i) AOM and DSS plus vehicle; ii) AOM, DSS and 2 mg/kg PT; and iii) AOM, DSS and 4 mg/kg PT. It was demonstrated that the histological acuteness of AOM/DSS-induced CAC was significantly reduced following the administration of PT, resulting in decreased NF-B p65 expression levels via a blockade of phosphorylation and subsequent degradation of inhibitor of B- (IB). Furthermore, PT administration appeared to enhance the process of carcinogenesis via the downregulation of the antiapoptotic proteinsBcl-2 and Bcl-extra large, mediated by inhibition of NF-B activation. Apoptosis and caspase-3 expression were markedly increased in the PT-treated group. These findings indicate that PT inhibits IB phosphorylation and NF-B activation, resulting in the initiation of apoptosis and the eventualsuppression of CAC development. The beneficial effects of PT treatment observed in the experimental CAC model indicate the potential chemopreventive and therapeutic role of PT in CAC.

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05:06

Africans Do Not Want to be Used as Guinea Pigs for Untested American GMO Technology Health Impact News

African_Farmer_watering_maize_1200x600

Africans must not be used as guinea pigs for untested high-risk new GM technology

by GMWatch.org

The Alliance for Food Sovereignty in Africa has called for an immediate ban on the importation into South Africa of Monsantos high-risk second-generation gene-silencing GM maize destined for human consumption.

In an open letter to African biosafety regulators, AFSA rejects and condemns US corporation Monsantos plan to exploit millions of Africans as unwitting human guinea pigs for their latest genetic engineering experiment. AFSA also condemns the IITA (International Institute of Tropical Agriculture) field trial application in Nigeria using this same risky technology to produce GM cassava for the agro-fuels industry.

These GM applications target staple foods of maize and cassava, eaten by many millions of Africans every day. AFSAs open letter states:

Scientists have reported that the untested gene-silencing effect is able to cross over into mammals and humans, and affect their genetic makeup with unknown potential negative consequences, and have called for long-term animal testing and stronger regulation before this goes ahead.

AFSA demands that while these risks remain, the introduction of this untested RNAi technology be unequivocally banned by all member states of the African Union. Regulators in South Africa and Nigeria are urged to reject the importation of this GM maize, and the field-testing of GM cassava. These grave threats to the health and well being of African peoples must be recognized, understood and resisted.

AFSA campaigns for food sovereignty the right of peoples to healthy and culturally appropriate food produced through ecologically sound and sustainable methods, and their right to dene their own food and agriculture systems. It urges African policymakers to reject genetic engineering and support the transition to agroecology as the sustainable future of farming in Africa.

Source: African Centre for Biodiversity
https://acbio.org.za/open-letter-...

04:55

Parthenolide demonstrated activity against stage V gametocytes and multiple mosquito-specific stages of the Plasmodium parasite. GreenMedInfo

PMID:  Antimicrob Agents Chemother. 2016 Apr ;60(4):2108-17. Epub 2016 Mar 25. PMID: 26787692 Abstract Title:  The Nonartemisinin Sesquiterpene Lactones Parthenin and Parthenolide Block Plasmodium falciparum Sexual Stage Transmission. Abstract:  Parthenin and parthenolide are natural products that are closely related in structure to artemisinin, which is also a sesquiterpene lactone (SQL) and one of the most important antimalarial drugs available. Parthenin, like artemisinin, has an effect onPlasmodiumblood stage development. We extended the evaluation of parthenin as a potential therapeutic for the transmissible stages ofPlasmodium falciparumas it transitions between human and mosquito, with the aim of gaining potential mechanistic insight into the inhibitory activity of this compound. We posited that if parthenin targets different biological pathways in the parasite, this in turn could pave the way for the development of druggable compounds that could prevent the spread of artemisinin-resistant parasites. We examined parthenin's effect on male gamete activation and the ookinete-to-oocyst transition in the mosquito as well as on stage V gametocytes that are present in peripheral blood. Parthenin arrested parasite development for each of the stages tested. The broad inhibitory properties of parthenin on the evaluated parasite stages may suggest different mechanisms of action between parthenin and artemisinin. Parthenin's cytotoxicity notwithstanding, its demonstrated activity in this study suggests that structurally related SQLs with a better safety profile deserve further exploration. We used our battery of assays to test parthenolide, which has a more compelling safety profile. Parthenolide demonstrated activity nearly identical to that of parthenin againstP. falciparum, highlighting its potential as a possible transmission-blocking drug scaffold. We discuss the context of the evidence with respect to the next steps toward expanding the current antimalarial arsenal.

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04:42

Girl Commits Suicide After Gardasil Vaccine Destroys Her Life Health Impact News

maddie-gardasil-death2

Maddie died on June 24, 2017

Health Impact News

The VAXXED team recently interviewed a mother who tells about how her daughter took her own life after suffering from the Gardasil vaccine for several years.

A doctor in their church recommended the HPV vaccine to them. After receiving the second Gardasil vaccine at the age of 15, she became very lethargic and could hardly get out of bed, suffering from horrible headaches. They had to black out the light in her room because of the pain it caused, and she did not leave her bed for days.

Prior to the vaccine, she was healthy and active, and rarely missed a day of school.

The family had to hire a teacher to teach her sophomore year of high school since she could not leave her home.

After starting a very strict diet that an alternative health practitioner recommended, the family had some hope that she could recover.

But she battled depression, and it was learned from her journals after her death that she had a constant buzzing sound in her head. She had tried every anti-depressant drug on the market without success.

Listen to the whole tragic interview below.

Comment on this article at VaccineImpact.com.

gardasil_collage-3

Young women whose lives were destroyed by the HPV vaccine.

California Nurse Gives Gardasil Vaccine to Own Daughter who Develops Leukemia and Dies

...

04:38

parthenolide can be developed as a drug used in combination therapy against melanoma. GreenMedInfo

PMID:  Oncotarget. 2016 Feb 23 ;7(8):9026-40. PMID: 26824319 Abstract Title:  Parthenolide induces MITF-M downregulation and senescence in patient-derived MITF-M(high) melanoma cell populations. Abstract:  The activity of the M isoform of microphthalmia-associated transcription factor (MITF-M) has been attributed to regulation of differentiation, proliferation, survival and senescence of melanoma cells. MITF expression was shown to be antagonized by the activation of transcription factor NF-B. Parthenolide, an inhibitor of NF-B, has not been yet reported to affect MITF-M expression. Our results obtained in patient-derived melanoma cell populations indicate that parthenolide efficiently decreases the MITF-M level. This is neither dependent on p65/NF-B signaling nor RAF/MEK/ERK pathway activity as inhibition of MEK by GSK1120212 (trametinib) and induction of ERK1/2 activity by parthenolide itself do not interfere with parthenolide-triggered depletion of MITF-M in both wild-type BRAF and BRAF(V600E) melanoma populations. Parthenolide activity is not prevented by inhibitors ofcaspases, proteasomal and lysosomal pathways. As parthenolide reduces MITF-M transcript level and HDAC1 protein level, parthenolide-activated depletion of MITF-M protein may be considered as a result of transcriptional regulation, however, the influence of parthenolide on other elements of a dynamiccontrol over MITF-M cannot be ruled out. Parthenolide induces diverse effects in melanoma cells, from death to senescence. The mode of the response to parthenolide is bound to the molecular characteristics of melanoma cells, particularly to the basal MITF-M expression level but other cell-autonomous differences such as NF-B activity and MCL-1 level might also contribute. Our data suggest that parthenolide can be developed as a drug used in combination therapy against melanoma when simultaneous inhibition of MITF-M, NF-B and HDAC1 is needed.

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04:31

New: CIA Agent Whistleblower Risks All To Expose The Shadow Government Geoengineering Watch

GeoengineeringWatch.org 6675Dane Wigington GeoengineeringWatch.org Kevin Shipp (author of "From The Company Of Shadows") was a decorated CIA officer who refused to look the other way in regard to government criminality and cover-up. At a very important public awareness event, held by GeoengineeringWatch.org in Northern California, on July 28th, 2017, Mr. Shipp presented a shocking and compelling presentation on numerous, horrific and ongoing

04:25

The Criminal Intent of Compulsory Vaccination Vaxxter

by Dr. Stefanie Hillinger, Independent Research Center, The Dayeng Foundation Are compulsory vaccinations in Italy and France mass murder by the System? No argument can justify this. Putting something into the body of another human being under...

The post The Criminal Intent of Compulsory Vaccination appeared first on Vaxxter.

04:23

Girl Commits Suicide After Gardasil Vaccine Destroys Her Life Vaccine Impact

maddie-gardasil-death2

Maddie died on June 24, 2017

Health Impact News

The VAXXED team recently interviewed a mother who tells about how her daughter took her own life after suffering from the Gardasil vaccine for several years.

A doctor in their church recommended the HPV vaccine to them. After receiving the second Gardasil vaccine at the age of 15, she became very lethargic and could hardly get out of bed, suffering from horrible headaches. They had to black out the light in her room because of the pain it caused, and she did not leave her bed for days.

Prior to the vaccine, she was healthy and active, and rarely missed a day of school.

The family had to hire a teacher to teach her sophomore year of high school since she could not leave her home.

After starting a very strict diet that an alternative health practitioner recommended, the family had some hope that she could recover.

But she battled depression, and it was learned from her journals after her death that she had a constant buzzing sound in her head. She had tried every anti-depressant drug on the market without success.

Listen to the whole tragic interview below.

gardasil_collage-3

Young women whose lives were destroyed by the HPV vaccine.

California Nurse Gives Gardasil Vaccine to Own Daughter who Develops Leukemia and Dies

Infant Accidentally Vaccinated with Gardasil Mother Blamed for Vaccine Injuries and Baby Medically Kidnapped

...

03:51

Skin Problems and Their Treatments Doom and Bloom (TM)

SKIN PROBLEMS AND TREATMENTS Treating medical problems in a remote homestead or after a disaster wont always be about gunshot wounds and broken bones.  Sometimes, little things can make people miserable and affect their ability to contribute to group efforts. Skin inflammation, called dermatitis, is one of the issues that a caregiver cant ignore if the group is going to function at 100% efficiency. This condition has various causes and varies in appearance from case to case, although most present

[... Continue Reading]

03:02

Targeting thioredoxin reductase by parthenolide contributes to inducing apoptosis of HeLa cells. GreenMedInfo

PMID:  J Biol Chem. 2016 May 6 ;291(19):10021-31. Epub 2016 Mar 21. PMID: 27002142 Abstract Title:  Targeting Thioredoxin Reductase by Parthenolide Contributes to Inducing Apoptosis of HeLa Cells. Abstract:  Parthenolide (PTL), a major active sesquiterpene lactone from the herbal plant Tanacetum parthenium, has been applied in traditional Chinese medicine for centuries. Although PTL demonstrates potent anticancer efficacy in numerous types of malignant cells, the cellular targets of PTL have not been well defined. We reported here that PTL interacts with both cytosolic thioredoxin reductase (TrxR1) and mitochondrial thioredoxin reductase (TrxR2), two ubiquitous selenocysteine-containing antioxidant enzymes, to elicit reactive oxygen species-mediated apoptosis in HeLa cells. PTL selectively targets the selenocysteine residue in TrxR1 to inhibit the enzyme function, and further shifts the enzyme to an NADPH oxidase to generate superoxide anions, leading to reactive oxygen species accumulation and oxidized thioredoxin. Under the conditions of inhibition of TrxRs in cells, PTL does not cause significant alteration of cellular thiol homeostasis, supporting selective target of TrxRs by PTL. Importantly, overexpression of functional TrxR1 or Trx1 confers protection, whereas knockdown of the enzymes sensitizes cells to PTL treatment. Targeting TrxRs by PTL thus discloses an unprecedented mechanism underlying the biological activity of PTL, and provides deep insights to understand the action of PTL in treatment of cancer.

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03:00

Vaccine Mandates: The Spite House Well All Have to Live In The Thinking Moms' Revolution

August 23, 2017 Today were doing something a little different. Jill Boman, a Facebook friend of mine, posted a comment linking spite houses and vaccine mandates, and it was so good I asked her to write us a blog on Continue reading

The post Vaccine Mandates: The Spite House Well All Have to Live In appeared first on The Thinking Moms' Revolution.

02:44

Parthenolide exerts cytotoxic effects on breast cancer stem-like cells. GreenMedInfo

PMID:  Cell Death Dis. 2016 Apr 14 ;7:e2194. Epub 2016 Apr 14. PMID: 27077810 Abstract Title:  Parthenolide and DMAPT exert cytotoxic effects on breast cancer stem-like cells by inducing oxidative stress, mitochondrial dysfunction and necrosis. Abstract:  Triple-negative breast cancers (TNBCs) are aggressive forms of breast carcinoma associated with a high rate of recidivism. In this paper, we report the production of mammospheres from three lines of TNBC cells and demonstrate that both parthenolide (PN) and its soluble analog dimethylaminoparthenolide (DMAPT) suppressed this production and induced cytotoxic effects in breast cancer stem-like cells, derived from dissociation of mammospheres. In particular, the drugs exerted a remarkable inhibitory effect on viability of stem-like cells. Such an effect was suppressed by N-acetylcysteine, suggesting a role of reactive oxygen species (ROS) generation in the cytotoxic effect. Instead z-VAD, a general inhibitor of caspase activity, was ineffective. Analysis of ROS generation, performed using fluorescent probes, showed that both the drugs stimulated in the first hours of treatment a very high production of hydrogen peroxide. This event was, at least in part, a consequence of activation of NADPH oxidases (NOXs), as it was reduced by apocynin and diphenylene iodinium, two inhibitors of NOXs. Moreover, both the drugs caused downregulation of Nrf2 (nuclear factor erythroid 2-related factor 2), which is a critical regulator of the intracellular antioxidant response. Prolonging the treatment with PN or DMAPT we observed between 12 and 24h that the levels of both superoxide anion and hROS increased in concomitance with the downregulation of manganese superoxide dismutase and catalase. In addition, during this phase dissipation of mitochondrial membrane potential occurred together with necrosis of stem-like cells. Finally, our results suggested that the effect on ROS generation found in the first hours of treatment was, in part, responsible for the cytotoxic events observed in the successive phase. In conclusion, PN and DMAPT markedly inhibited viability of stem-like cells derived from three lines of TNBCs by inducing ROS generation, mitochondrial dysfunction and cell necrosis.

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02:28

First Of Its Kind Study Shows Undeniable Evidence Cannabis Can Cure Opioid Addiction Natural Blaze

By Justin Gardner

As the opioid epidemic rages on, those professing to seek solutions are willfully ignoring one of the most promising treatments medical cannabis. When the Comprehensive Addiction & Recovery Act (CARA) was being debated in 2016, amendments to study medical cannabis were stripped out.

This happened despite studies showing that medical cannabis eases neuropathic pain, and the governments own National Institutes of Health stating, Medical marijuana products may have a role in reducing the use of opioids needed to control pain.

There were also studies showing that deaths from opioids plummet in states with legal cannabis, and that 80 percent of cannabis users give up prescription pills. A Feb. 2017 study confirmed that opioid dependence and overdoses dropped significantly in medical cannabis states.

In January 2017, the...

02:17

Parthenolide induces apoptosis and autophagy-mediated growth inhibition in HeLa cells. GreenMedInfo

PMID:  Biotechnol Lett. 2016 Aug ;38(8):1251-60. Epub 2016 Apr 21. PMID: 27099069 Abstract Title:  Parthenolide induces apoptosis and autophagy through the suppression of PI3K/Akt signaling pathway in cervical cancer. Abstract:  OBJECTIVE: To investigate the effect of parthenolide on apoptosis and autophagy and to study the role of the PI3K/Akt signaling pathway in cervical cancer.RESULTS: Parthenolide inhibits HeLa cell viability in a dose dependent-manner and was confirmed by MTT assay. Parthenolide (6 M) induces mitochondrial-mediated apoptosis and autophagy by activation of caspase-3, upregulation of Bax, Beclin-1, ATG5, ATG3 and down-regulation of Bcl-2 and mTOR. Parthenolide also inhibits PI3K and Akt expression through activation of PTEN expression. Moreover, parthenolide induces generation of reactive oxygen species that leads to the loss of mitochondrial membrane potential.CONCLUSION: Parthenolide induces apoptosis and autophagy-mediated growth inhibition in HeLa cells by suppressing the PI3K/Akt signaling pathway and mitochondrial membrane depolarization and ROS generation. Parthenolide may be a potential therapeutic agent for the treatment of cervical cancer.

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02:12

9 Natural Arthritis Remedies Natural Blaze

By Adonia Dennis, Natural Blaze

Arthritis is a disease that can cause severely painful symptoms in joints throughout the entire body. According to the Arthritis Foundation, over 50 million American adults have been diagnosed with some form of arthritis, which accounts for more than 20% of all people of the age of 18 in the United States. In addition to these statistics, at least 300,000 children in the country have some form of arthritis or a rheumatic disease.

This particular disease is the primary cause of disability in the United States and many other countries in the world. The Centers for Disease Control and Prevention reports that the most common types of arthritis diagnosed in the United States include osteoarthritis and rheumatoid arthritis, with gout, fibromyalgia, and lupus also being fairly common.

While the majority of arthritis types are not curable diseases, the symptoms they cause can be controlled, and the progression of the diseases can be slowed down with appropriate treatment options.

...

02:09

TODAY: last day to save on conference hotel room Fluoride Action Network

Today, August 23rd, is the last day to book your hotel room for the the 6th Citizens Conference on Fluoride at a significantly discounted rate!

CLICK HERE TO RESERVE YOUR ROOM

The conference will take place at:

Hyatt Regency Hotel, Crystal City, Virginia
on September 1618, 2017

The hotel is terrific and each room has WiFi.  Its located in a safe area with several restaurants close-by.  Its also close to the Washington DCA airport where there is a free hotel shuttle bus, and its just a few quick metro (subway) stops from Union Station in Washington, D.C. see more on our conference Overview page.

Important Links

02:06

Parthenolide ameliorates diabetes-induced behavioural deficit, neurotransmitter imbalance and neuroinflammation in type 2 diabetes rat model. GreenMedInfo

PMID:  Neuromolecular Med. 2017 Mar ;19(1):101-112. Epub 2016 Aug 23. PMID: 27553015 Abstract Title:  Parthenolide, an NF-B Inhibitor Ameliorates Diabetes-Induced Behavioural Deficit, Neurotransmitter Imbalance and Neuroinflammation in Type 2 Diabetes Rat Model. Abstract:  Diabetes is associated with behavioural and neurochemical alterations. In this manuscript, we are reporting the beneficial effects of parthenolide, an NF-B inhibitor on behavioural and neurochemical deficits in type 2 diabetic rat model. Diabetes was induced by high-fat diet followed by low dose of streptozotocin (35 mg/kg). Elevated plus maze, open-field, MWM and passive avoidance test paradigm were used to assess behavioural and cognitive deficits. Three-week treatment of parthenolide (0.25 and 0.50 mg/kg; i.p.) attenuated diabetes-induced alteration in cognitive function in Morris water maze and passive avoidance test. Anxiety-like behaviour was also reduced by parthenolide treatment. Moreover, TNF- and IL-6 levels were significantly decreased in cortex and hippocampus of parthenolide-treated rats. Three-week parthenolide treatment also toned down the alteration of GABA and glutamate homoeostasis. Results of this study corroborate the involvement of neuroinflammation in the development of behavioural and neurochemical deficits indiabetic animals and point towards the therapeutic potential of parthenolide in diabetes-induced alteration of learning, memory and anxiety behaviour.

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01:55

Florida Teen Suffers Debilitating Narcolepsy Vaxxter

Ruth Hamper: If he goes down, we need to get to him right away to keep him safe. A Florida teenager has been diagnosed with a rare disease that causes him to fall asleep for...

The post Florida Teen Suffers Debilitating Narcolepsy appeared first on Vaxxter.

01:40

Sign the Statement to Defend Kratom Natural Blaze

By Dave Herman
Chairman, American Kratom Association

On August 31, 2016, the Drug Enforcement Administration (DEA) rocked the kratom world with their now infamous Notice of Intent for Emergency Scheduling of kratom as a Schedule I controlled substance.

That was truly the day that the federal government declared war on kratom and war on every one of us who believe that consumers should have the freedom to make their own decisions about how to manage their health and well-being.

But the truth is, the DEA and the FDA picked a fight with people who were unwilling to be trampled upon by the unfair and arbitrary criminalization of kratom and of the basic freedoms we have every right to enjoy.

The attempt to schedule kratom ignited a grassroots movement that continues to this very day, and I am pleased to report that we are growing stronger and stronger in our continued fight against the demonization of kratom by these federal bureaucrats.

On August 31, 2017, I intend to deliver a statement to the Presidential Commission on Combating Drug Addiction and the Opioid Crisis asking the government to recognize the numerous studies that show kratom can be used as an alternative pain management option, but kratom can also be a potentially effective Reverse-Gateway out of opioid addiction.

I am hoping that you will sign up to be an official co-signer on the American Kratom Association Statement to the Opioid Commission.

Please click on the link below and review the Statement to the Commission.  If you agree with the Statement, and I hope you will, then I need you to add your name as an official co-signer of the statement.

...

01:30

Former MD Anderson researcher objects to retraction of his paper Retraction Watch

A cell biology journal has retracted a 2016 paper after an investigation revealed that the corresponding author failed to include two co-authors and acknowledge the funding source. According to the retraction notice, the Journal of Cellular Physiology retracted the paper after the University of Texas, MD Anderson Cancer Center found that last author Jin Wang []

The post Former MD Anderson researcher objects to retraction of his paper appeared first on Retraction Watch.

00:57

The Science of Fluoride Flipping Fluoride Action Network

Chapel Hill, NC So much of what happens inside cells to preserve health or cause disease is so small or time-sensitive that researchers are just now getting glimpses of the complexities unfolding in us every minute of the day.

UNC School of Medicine researchers have discovered one such complexity a previously hidden mode of RNA regulation vital for bacterial defence against toxic fluoride ions.

Published in the journal Nature Chemical Biology, the discovery opens a new research avenue for developing drugs that target RNA genetic molecules important for various biological processes, including how genes are regulated.

Much research to find the underpinnings of health and disease has rightfully focused on proteins, but different forms of RNA have functions were just beginning to understand, said Dr.Qi Zhang, senior author and assistant professor of biochemistry and biophysics. Our NMR technique is helping us learn more than ever before.

In 2014, Zhang and colleagues developed a new way to use nuclear magnetic resonance (NMR) imaging to show the shape and motion of RNA at the atomic level over time. This was crucial because RNA is often short-lived and sparsely populated in cells at any given time. The amount of RNA changes over short bursts of time depending on which one of its various roles it is fulfilling. Yet, until now, structural biologists have only visualized RNA as a series of snapshots. Zhangs technique enables new ways of visualizing RNA, down to its atoms.

We need this atomic level view because every atomic interaction is important to human health, said Zhang, who is also a member of the UNC Lineberger Comprehensive Cancer Center. Scientists have developed similar approaches that work well for proteins and we needed this for RNA, which is crucial for understanding how an RNA serves as a control switch for gene expression.

In their latest work, Zhang and colleagues studied riboswitches a class of noncoding RNAs that are not translated from DNA into proteins. Rather, riboswitches control gene expression in response to specific cellular cues. Many bacteria rely on these cues and controls to regulate fundamental cellular function. These switches have been important models for the scientific communitys basic understanding of RNA architecture and ligands molecules such as drug compounds. Riboswitches have emerged as targets for a new class of very much needed antibacterial drugs.

Heres the prevailing wisdom of how these riboswitches work: when a cell produces a metabolite or encounters a toxin to a certain level, a sensor on the riboswitch detects this, reshapes the switchs three-dimensional structure, and sends a signal to turn the responsible gene circuit on or off. This model has been shown in a...

00:45

Some Thoughts About Conferences Mad In America

Editors note: for personal and family reasons the author has chosen to publish under a pen name. 

It might sound strange, as I still get told a lot how young I seem to be, but Im fed up with attending conferences. At each of them we talk and analyze psychiatry as if we have to justify to ourselves that were standing on the right side, or as if were waiting for someone to give us the right to do what we do. We repeat again and again whats wrong with psychiatry, how we need different forms of support, that a social change is needed, etc. From conference to conference I keep asking myself, why all this?

Without judging the motivation of people presenting and speaking at conferences and without forgetting that ex-users of psychiatry are still struggling to be heard and conferences are there to give voice to those with lived experience Id like to ask the question: can we achieve more with these conferences than generating knowledge and touching peoples hearts?

Why is it important to ask such a question? It is important because we are judging a miserable situation in society. We should therefore ask ourselves if our kind of disputation, which is mainly critical and analytical, fits with the judged grievance. In other words: are we preparing the ground for change or are we marking time?

We all know whats going wrong in psychiatry and what should be changed. Weve known for decades. Thats the reason I dont think well make much difference with further analyses of this kind. What we need is to work out strategies for how to use our critique to change and abolish psychiatry. We need to analyze where the cracks in psychiatry are, and take action with our critique at exactly those points to knock over the monopoly on truth that psychiatry has had. We need to sit with each other and discuss how we can build up places to support each other and be who we are, no matter what anyone else is saying about who and what we are. Reaching out for more people to join our movement is quite important, but it feels secondary to me. It feels more urgent to ask: where and when will we start to attack?

This step away from defense to attack takes a lot of courage and strength. We have to pit ourselves against a recognized system but one that is s...

00:37

Parthenolide reduces gene transcription of prosurvival mediators in U937 cells. GreenMedInfo

PMID:  Exp Oncol. 2017 03 ;39(1):30-35. PMID: 28361855 Abstract Title:  Parthenolide reduces gene transcription of prosurvival mediators in U937 cells. Abstract:  : In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway or-catenin have been considered.AIM: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN and-catenin genes.MATERIALS AND METHODS: The U937 cells were treated with PTL at concentrations of 4M (IC25) or 6 M (IC50) and with OPN siRNA for MTT assay and colony forming assay. Western blot analysis using antibodies against OPN was performed with lysates of PTL-treated cells. Quantitative real-time polymerase chain reaction was performed using primers for OPN siRNA, RelB, AKT1, mTOR, PTENand -catenin.RESULTS: PTL reduces OPN protein level and down-regulates RelB mRNA in U937 cell line. Suppression of OPN with siRNA increases the cytotoxic effects of PTL. Also, mRNA expression of AKT1, mTOR, PTEN, and-catenin decreases with PTL or OPN siRNA.CONCLUSION: Sensitivity of U937 cells to PTL can be associated with the reduction in expression of prosurvival mediators.

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00:25

Parthenolide may be a promising component for bone regeneration in periodontitis treatment. GreenMedInfo

PMID:  J Interferon Cytokine Res. 2017 Aug 22. Epub 2017 Aug 22. PMID: 28829282 Abstract Title:  Parthenolide Promotes Differentiation of Osteoblasts Through the Wnt/-Catenin Signaling Pathway in Inflammatory Environments. Abstract:  Periodontitis is a progressive inflammatory disease initiated by bacterial biofilm adhering to the tooth surface. If left untreated, periodontitis may lead to tooth loss and destruction of the alveolar bone. Regaining the lost alveolar bone is a clinical challenge because of the limited differentiation ability of osteoblasts in inflammatory environments. We have previously shown the anti-inflammatory and antiosteoclastogenic activities of parthenolide (PTL) in human periodontal ligament-derived cells by inhibiting nuclear factor kappa B (NF-B) signaling, indicating its potential for periodontitis treatment. In this study, we further examined whether PTL could stimulate differentiation of osteoblasts from human alveolar bone in inflammatory conditions and investigated the involvement of the Wnt/-catenin signaling pathway during thisprocess. The results showed that PTL significantly stimulated alkaline phosphatase activity, mineralization nodule formation, and osteogenesis-related gene/protein expression of osteoblasts under the stimulation of tumor necrosis factor- (TNF-). In addition, PTL inhibited the NF-B/p50 pathwayand resisted the inhibition of Wnt/-catenin signaling induced by TNF-. Our results indicate that the stimulatory effect of PTL on the differentiation of osteoblasts in inflammatory environments may involve the activation of the Wnt/-catenin signaling pathway, and PTL may be a promising component for bone regeneration in periodontitis treatment.

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Wednesday, 23 August

23:49

Vaccine Disasters Governments Want You To Forget World Mercury Project

By Jon Rappoport

Parents who question vaccines are crazy? Really? Parents who claim to discover vaccine-dangers are crazy? Really?

Official history is a curious thing. Whole sections of fact disappear from the record.

When I was researching my first book, AIDS INC., in 1987-88, I explored various forms of immune-system suppression, in order to show that HIV was not the real story.

I looked into vaccines, for example. Here is an excerpt from AIDS INC. Keep in mind that my research, at the time, ended in 1988. This excerpt reports on vaccines disasters and statements about vaccines made by several authors.

It is forgotten history:

  • Based on the only U.S. findings on adverse DPT [diphtheria-pertussis-tetanus vaccine] reactions, an FDA-financed study at the University of California, Los Angeles, one out of every 350 children will have a convulsion; one in 180 children will experience high-pitched screaming [can indicate brain damage]; and one in 66 will have a fever of 105 degrees or more. (Jennifer Hyman, Democrat and Chronicle, Rochester, New York, special supplement on DPT, dated April, 1987.)
  • A study undertaken in 1979 at the University of California, Los Angeles, under the sponsorship of the Food and Drug Administration, and which has been confirmed by other studies, indicates that in the U.S.A. approximately 1,000 infants die annually as a direct result of DPT vaccinations, and these are classified as SIDS (Sudden Infant Death Syndrome) deaths. These represent about 10 to 15% of the total number of SIDS deaths occurring annually in the U.S.A. (between 8,000 and 10,000 depending on which statistics are used). (Leon Chaitow, Vaccination and Immunization, CW Daniel Company Limited, Saffron Walden, Essex, England, 1987.)
  • Assistant Secretary of Health Edward Brandt, Jr., MD, testifying before the U.S. Senate Committee on Labor and Human Resources, rounded figures off to 9,000 cases of convulsions, 9,000 cases of collapse, and 17,000 cases of high-pitched screaming for a total of 35,000 acute neurological reactions occurring within forty-eight hours of a DPT shot among Americas children every year. (DPT: A Shot in the Dark, by Harris L. Coulter and Barbara Loe Fischer, Harcourt Brace Jovanovich.)
  • Barker and Pichichero, in a prospective study of 1232 children in Denver, Colorado, found after DTP that only 7% of those vaccinated were free from untoward reactions, which included pyrexia [fever] (53%), acute behavioral changes (82%), prolonged screaming (13%), and listlessness, anorexia and vomiting. 71% of those receiving second injections of DTP experienced two or more of the reactions monitored. (Lancet, May 28, 1983, p. 1217)
  • the swine-flu vaccination program was one of its (CDC) greatest blundersIt all began in 1976 when CDC scientists saw that a virus involved in a f...

23:48

AQMD: Not enough data to prove safety of deadly toxic chemical used at Torrance refinery Fluoride Action Network

Southern Californias air quality watchdog has become the latest regulatory agency to cast doubt on the safety of a potentially deadly chemical used at the Torrance refinery, concluding there is not enough evidence to dismiss concerns that a toxic cloud could form and kill or injure tens of thousands.

South Coast Air Quality Management District staff members came to what they described as an initial conclusion while reviewing data provided by refinery owner PBF Energy via its subsidiary, the Torrance Refining Co.

The agency is exploring whether to create a rule that would phase out the use of hydrofluoric acid, especially in the massive quantities used in Torrance.

A modified form of the acid is used in Torrance and at Valeros Wilmington refinery, the only two in the state that use hydrofluoric acid.

The MHF is supposed to inhibit the formation of the cloud; critics have questioned whether that would actually occur.

Meanwhile, the AQMD also questioned whether existing safety measures are sufficient to prevent a catastrophic HF release of the sort federal officials said almost occurred in February 2015, when a massive explosion ripped through the plant then owned by ExxonMobil.

Data Lacking

Staffs initial conclusion is that the testing/modeling information provided by Torrance Refining Company did not sufficiently demonstrate MHF would not flash atomize and form dense HF cloud, reads a report posted on the agencys website earlier this month.

Even existing mitigations do not guarantee adequate protection in (an) unplanned event such as a major accident or earthquake causing equipment failure, the report added.

A full report is scheduled to be presented today at the fifth meeting of a working group in Diamond Bar that is looking at the new rule.

The AQMD indicated that preliminary language on the new rule and a staff report would be prepared ahead of a September working group meeting.

This is the first time the AQMD has sided with such agencies as the federal Chemical Safety Board and Environmental Protection Agency that also have cast doubt on the safety of MHF and the effectiveness of safety systems meant to protect the community.

PBF Responds

But the report was met with a swift response by PBF Energy, which fired off a lette...

23:41

Albuquerque: Fluoride could be added to metros drinking water; final vote tonight Fluoride Action Network

Citizens will be able to weigh in on a proposed plan that would once again add more fluoride to drinking water in Albuquerque.

Members of the water authority board are expected to make a final vote on a plan to spend more than $200,000 for equipment and facilities to allow the city to resume fluoridation.

Now, the debate over adding fluoride to the metros water is heating up again. While officials say it would prevent tooth decay, there are some who believe fluoride is a toxic chemical.

Back in the 1970s, citizens approved the fluoridation of drinking water. In 2011, the Albuquerque Bernalillo County Water Utility Authority halted those operations as they waited for new federal fluoride level recommendations to eventually be released in 2015.

What is being voted on Wednesday night is a one-time cost of $260,000 coming from capital funds, which would pay for the construction of a new facility at the San Juan Chama Plant. Officials say it would cost $270,000 a year for operation and maintenance costs.

Officials also say it would add fluoride to the already existing levels of in the water, bringing it to .07 milligrams, the most beneficial level for residents.

Supporters of supplemental fluoridation are concerned about the dental health in our community, particularly in underserved populations who may not be able to get to a dentist regularly. They see this as a way of helping to improve the dental health for those populations, David Morris, Public Affairs Manager with the Water Authority said.

The proposed plan doesnt come without controversy. The Department of Health says theyve received opposition from people saying that fluoride causes health problems such as Alzheimers disease.

The final vote is expected to happen at Vincent E. Griego Council Chambers at 5 p.m.

It is open to public comment. If you are unable to attend, you can email comments to ABQFluoride2017@gmail.com.

*Original article online at http://krqe.com/2017/08/23/fluoride-could-be-added-to-metros-drinking-water-final-vote-tonight/

23:36

Port Angeles: Council Candidates Discuss Fluoridation Issue Fluoride Action Network

PORT ANGELES Two candidates vying for a seat on the Port Angeles City Council agreed Tuesday the city should eschew second-class status and encourage economic development and weighed in on an avigation easement for William R. Fairchild Memorial Airport.

Lindsey Schromen-Wawrin and Artur Wojnowski disagreed on how to answer a Nov. 7 advisory vote on fluoridated drinking water, annexation and other issues during a Port Angeles Business Association forum at Joshuas Restaurant.

Wawrin and Wojnowski are running in the November general election for Port Angeles City Council Position 3, a four-year nonpartisan position held by Mayor Patrick Downie. Downie is not running for re-election.

Schromen-Wawrin, a Port Angeles native and a lawyer, was a Democratic Party precinct committeeman and recent president of the Clallam County Bar Association.

Wojnowski, a Chicago native who was raised in a Polish household, is maintenance manager for the Dungeness Meadows Home Owners Association.

I dont believe that we should be fluoridating the water, Wojnowski said.

The City Council voted 4-3 last August to stop adding fluoride to the municipal water supply, ending a 10-year-old practice.

Downie, who voted in December 2015 to continue fluoridation, cast the deciding vote last summer.

I believe that we dont know how much [fluoride] we all need to intake, Wojnowski said.

And given the fact that none of us really know how much is required, personally I dont think we should just be blanketing it. But again, thats for everyone to decide on their own.

Schromen-Wawrin said he had a more nuanced view on fluoride, saying the chemical has been shown to improve oral health and that he uses fluoride in his daily dental regime.

Schromen-Wawrin said he would personally vote no on the advisory vote but listen to the will of the voters and act accordingly, provided he has assurances that the city would not put lead and arsenic in the water.

Weve been gridlocked for too long and we need to move on from this issue, Schromen-Wawrin said.

I think at this point this issue has been tossed to the will of the people, which is not necessarily the best way to decide public health matters.

So in that regard, I think Id vote with what the voters said, with my caveat that Im not going to be putting lead and arsenic in the water supply in a knowing and intentional way.

Wojnowski said he will oppose fluoridation regardless of the advisory vote.

People can voice their opinion voting, yes, but people who elected me would basically be voting for my stance as it is currently, Wojnowski said.

So I would stick to the no vote.

Each candidate was given three minutes to make an opening statement, two minutes to answer audience questions and two minutes to make a closing statement.

The 53-minute forum was moderated by PABA Vi...

Saturday, 19 August

10:05

Penile Itching: Causes, Home Treatments and When to See a Doctor Healthy and Natural World

An itchy penis can be a very irritating problem for men and can cause an uncontrollable urge to scratch the genital region. Penile itching is usually caused by a fungal or bacterial infection. However, treating an itchy penis can be difficult because the dark warm environment of the area at the top of the legs and groin causes germs to quickly multiply. Because of that, conditions causing itching in the male private parts can quickly spread to the scrotum, inner thigh, and groin.

There are many natural ways to treat itching around the male genitalia. Very often, better personal hygiene in the groin area is the first step to stop itch-causing infections getting worse. However, if the penile itching is because of fungi, lice, yeast infection, or an inflammatory skin condition, then some home remedies will help to get rid of the problem. Ingredients like tea tree oil, coconut oil, witch hazel, and apple cider vinegar help to both soothe the irritating itch and kill off infection-causing germs around male privates.

Sometimes, the itchy patch of skin on your penis could be a symptom of a more serious condition like a sexually transmitted disease or pubic lice. Also, constant scratching around the groin area could cause a bacterial skin infection that can be harder to treat.

This article looks at the various causes and remedies for an itchy penis. You will also find out how to prevent penile itching becoming a chronic condition where the itch-scratch-itch cycle becomes difficult to stop.

Symptoms of Itching Genital Area

Itching can affect any part of the penis from the base of the organ to the foreskin and tip. Very often, the itchy skin on the male member will be accompanied by a rash or red patch of skin. Depending on its cause and severity, the itching could be mild to severe.

Dr. Melissa Conrad Stppler on MedicineNet says that inflammation of the penis shaft could also cause a burning sensation and discomfort.1 If a yeast or bacterial infection is the cause of the penile itch, then you may also experience discharge from the site of the infection. Some conditions also cause a buildup of a cheesy substance under the foreskin if you are not circumcised.

In cases where dermatitis or psoriasis are to blame for the itchy private parts, along with the severe itching, you will notice scaling skin or a pink or brown patch. If the penis itching is severe, constant scratching may cause the skin to crack and bleed.

Causes and Treatment of Penile Itching

Various conditions and ailments can cause itching around the pubic and genital area of men. Knowing the reasons behind the embarrassing itching between your legs can help to find the best home remedy to get relief from the urge to scratch.

Jock itch

Jock itch (tinea cruris) is a common reason for having intense itching around the penis. The warm, moist conditions of the groin as...

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